Acero Nuria, Manrique Jorge, Muñoz-Mingarro Dolores, Martínez Solís Isabel, Bosch Francisco
Departamento de CC Farmacéuticas y de la Salud, Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, 28660 Boadilla del Monte, Spain.
Departamento de Química y Bioquímica, Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, 28660 Boadilla del Monte, Spain.
Antioxidants (Basel). 2025 Feb 27;14(3):279. doi: 10.3390/antiox14030279.
is a plant known since ancient times mainly for the interest of its fruits. However, its leaves have traditionally been consumed as food in some regions of the Mediterranean basin and as a medicinal remedy. In this work, the phytochemical profile of this part of the plant, which is considered a bio-residue of viticultural processes, was analyzed (UHPLC-ESI(±)-QTOF-MS). Hydroxybenzoic acids, flavonols, and stilbenes are the main phenolic compounds identified. Its antioxidant and anti-inflammatory capacity were studied both in vitro and in cell culture. Grapevine leaves have a high capacity to scavenge free radicals, as well as to reduce oxidative stress induced by HO in the HepG2 cell line. On the other hand, the methanolic extract of these leaves is capable of inhibiting lipoxygenase, an enzyme involved in inflammatory responses, with an IC of 1.63 μg/mL. In addition, the extract showed potent inhibition of NO production in LPS-stimulated RAW 264.7 cells. These results pointed out leaves as a powerful functional food with a high content of biologically active compounds. The enhancement of these by-products can be highly beneficial to food systems and contribute to the development of sustainable agriculture.
是一种自古以来就因其果实而闻名的植物。然而，在一些地中海盆地地区，其叶子传统上被作为食物食用，也被用作药物。在这项工作中，对该植物这一部分（被认为是葡萄栽培过程中的生物残渣）的植物化学特征进行了分析（超高效液相色谱-电喷雾离子化（±）-四极杆飞行时间质谱）。羟基苯甲酸、黄酮醇和芪类是鉴定出的主要酚类化合物。对其抗氧化和抗炎能力进行了体外和细胞培养研究。葡萄叶具有很高的清除自由基能力，以及降低HepG2细胞系中由羟基自由基诱导的氧化应激的能力。另一方面，这些叶子的甲醇提取物能够抑制脂氧合酶（一种参与炎症反应的酶），其半数抑制浓度为1.63μg/mL。此外，该提取物在脂多糖刺激的RAW 264.7细胞中显示出对一氧化氮产生的有效抑制作用。这些结果表明葡萄叶是一种富含生物活性化合物的强大功能性食品。这些副产品的强化对食品系统非常有益，并有助于可持续农业的发展。
