Briend-Sutren M M, Le Maho S, Rommain M, Lambré C R
Unité 139 INSERM, Hopital H. Mondor, Créteil, France.
Agents Actions. 1990 Nov;31(3-4):308-12. doi: 10.1007/BF01997624.
An increased resistance of laboratory animals to pulmonary infections following per os administration of a glyco-proteic complex extracted from Klebsiella pneumoniae has been reported. This was associated with an increased phagocytic capacity of alveolar macrophages (AM). In this report, the effect of treating guinea pigs with this extract on the alveolar macrophage (AM) glycosidase machinery has been studied. AM were collected by bronchoalveolar lavage, the cells were pelleted by centrifugation and AM were purified by adherence on plastic dishes. Sialidase, beta-galactosidase, beta-glucuronidase and N-acetyl-beta-D glucosaminidase activities were measured in the AM homogenate. In order to evaluate an extracellular release of these enzymes, they were also assayed in the cell free lavage fluid. Lactic dehydrogenase (LDH) activity was assayed as a control for cell lysis. In treated animals, the total number of cells as well as the number of AM increased by 25% (ns). The protein concentration was slightly reduced in the cell homogenate and unchanged in the lavage fluid. The only significant change was a decreased sialidase activity, in AM homogenate (p less than or equal to 0.01) and in lavage fluids (ns). The LDH activity was not increased in the lavage fluids.
据报道，经口给予从肺炎克雷伯菌中提取的糖蛋白复合物后，实验动物对肺部感染的抵抗力增强。这与肺泡巨噬细胞（AM）的吞噬能力增强有关。在本报告中，研究了用这种提取物治疗豚鼠对肺泡巨噬细胞（AM）糖苷酶机制的影响。通过支气管肺泡灌洗收集AM，细胞通过离心沉淀，AM通过贴壁于塑料培养皿进行纯化。在AM匀浆中测量唾液酸酶、β-半乳糖苷酶、β-葡萄糖醛酸酶和N-乙酰-β-D-氨基葡萄糖苷酶的活性。为了评估这些酶的细胞外释放，还在无细胞灌洗液中对其进行了测定。测定乳酸脱氢酶（LDH）活性作为细胞裂解的对照。在治疗的动物中，细胞总数以及AM的数量增加了25%（无显著性差异）。细胞匀浆中的蛋白质浓度略有降低，灌洗液中的蛋白质浓度不变。唯一显著的变化是AM匀浆（p≤0.01）和灌洗液（无显著性差异）中唾液酸酶活性降低。灌洗液中的LDH活性没有增加。
