Abhishek Amar, Ansari Nasreen Ghazi, Shankhwar Satya Narayan, Jain Amita, Singh Vishwajeet
Department of Urology, King George׳s Medical University, Lucknow 226003, Uttar Predesh, India.
Metal Analysis Division, (CSIR)-Indian Institute of Toxicology Research, Post Box, No. 80, M.G. Marg, Lucknow 226001, Uttar Pradesh, India.
Bioinformation. 2014 Dec 31;10(12):716-20. doi: 10.6026/97320630010716. eCollection 2014.
The induced toxicity of three pesticides (alpha-Hexachlorocyclohexane: α-HCH; Parathion methyl:PM; Carbofuran: CN) in single and four possible combination on human keratinocyte cell line have been investigated. There was no significant change in toxicity (cyto and genotoxicity) on cell line exposed by individual pesticides except α-HCH. But, a synergistic effect was observed when we tested mixture of pesticides. The intracellular ROS and cytotoxicity assay revealed maximum reduction in cell viability (60%) was found in tri mixture of pesticides. All the possible combination of these pesticides demonstrated genotoxic activity in terms of olive tail moment and % tail DNA on cell line at low concentration. The order of toxicity was ranked as α-HCH+PM+CN>α- HCH+CN>PM+CN>α-HCH+PM. Our results call for more research to be undertaken in order to understand the mechanisms behind the synergy observed and quantify the extent of its environmental impacts.
研究了三种农药（α-六氯环己烷：α-HCH；甲基对硫磷：PM；克百威：CN）对人角质形成细胞系的单一毒性以及四种可能组合的诱导毒性。除α-HCH外，单一农药暴露的细胞系毒性（细胞毒性和遗传毒性）无显著变化。但是，当测试农药混合物时观察到协同效应。细胞内活性氧和细胞毒性测定显示，三种农药混合物使细胞活力降低最多（60%）。这些农药的所有可能组合在低浓度下对细胞系均表现出遗传毒性活性，以橄榄尾矩和尾DNA百分比衡量。毒性顺序为α-HCH+PM+CN>α-HCH+CN>PM+CN>α-HCH+PM。我们的研究结果呼吁开展更多研究，以了解所观察到的协同作用背后的机制，并量化其对环境影响的程度。
