Environmental Biotechnology Laboratory, Department of Life Sciences, University of Mumbai, Santacruz (E), Mumbai 400 098, India.
J Hazard Mater. 2011 Mar 15;187(1-3):333-40. doi: 10.1016/j.jhazmat.2011.01.034. Epub 2011 Jan 14.
In developing countries like India, there are many industrial areas discharging effluent containing large amount of polyaromatic hydrocarbon (PAH) which causes hazardous effect on the soil-water environment. The objective of this study was to isolate and characterize high-efficiency PAH-degrading microbial consortium from 3 decade old petrochemical refinery field located in Nagpur, Maharashtra with history of PAH disposal. Based on biochemical tests and 16S rDNA gene sequence analysis the consortium was identified as Sphingobacterium sp., Bacillus cereus and a novel bacterium Achromobacter insolitus MHF ENV IV with effective phenanthrene-degrading ability. The biodegradation data of phenanthrene indicates about 100%, 56.9% and 25.8% degradation at the concentration of 100mg/l, 250 mg/l and 500 mg/l respectively within 14 days. The consortium and its monoculture isolates also utilized variety of other hydrocarbons for growth. To best of our knowledge this is the first time that Achromobacter insolitus has been reported to mineralize phenanthrene effectively. GC-MS analysis of phenanthrene degradation confirmed biodegradation by detection of intermediates like salicylaldehyde, salicylic acid and catechol. All the results indicated that the microbial consortium have a promising application in bioremediation of petrochemical contaminated environments and could be potentially useful for the study of PAH degradation and for bioremediation purposes.
在印度等发展中国家，有许多工业区域排放含有大量多环芳烃 (PAH) 的废水，这对土壤-水环 境造成了危险的影响。本研究的目的是从位于马哈拉施特拉邦那格浦尔的一个有 30 年历史的石化炼油厂 地区分离和鉴定高效的 PAH 降解微生物群落，该地区有 PAH 处理的历史。根据生化测试和 16S rDNA 基因序列分析，该群落被鉴定为鞘氨醇单胞菌、蜡状芽孢杆菌和一种新型细菌 Achromobacter insolitus MHF ENV IV，具有有效降解菲的能力。菲的生物降解数据表明，在 14 天内，浓度分别为 100mg/l、250mg/l 和 500mg/l 时，降解率分别约为 100%、56.9%和 25.8%。该群落及其单培养物分离物也利用多种其他烃类物质进行生长。据我们所知，这是首次报道不动杆菌有效矿化菲。菲降解的 GC-MS 分析通过检测水杨醛、水杨酸和儿茶酚等中间产物证实了生物降解。所有结果表明，该微生物群落具有在石化污染环境的生物修复中具有广阔的应用前景，对于 PAH 降解的研究和生物修复目的可能具有潜在的用途。
