Second Department of Obstetrics and Gynecology, University of Athens, Aretaieion Hospital, Athens, Greece.
In Vivo. 2011 Jan-Feb;25(1):141-8.
Strong evidence implicates chronic intraamniotic inflammation in the etiology of preterm delivery. The purpose of this study was to determine whether amniotic fluid IL-1β, IL-10 and IL-18 concentrations in women undergoing mid-trimester amniocentesis can identify those at risk for preterm labor or preterm rupture of membranes.
A case-control study was conducted to compare mid-trimester concentrations of amniotic fluid IL-1β, IL-10 and IL-18 in women delivering at term or preterm. Out of 362 women included in the study, 38 presented with preterm labor. Thirty-eight women with term delivery, matched for chronological and gestational age served as controls. Women with abnormal fetal karyotypes or major anomalies were excluded. IL-1β, IL-10 and IL-18 concentrations were determined by ELISA. Conditional logistic regression was applied in the statistical analysis.
IL-1β was found to be positively and significantly associated with preterm delivery. Specifically, for every unit increase in IL-1β, women were on average 7.2 (OR: 7.2, CI: 1.94-26.77, p=0.003) times more likely to deliver preterm. IL-18 levels as well as gender were significantly associated with preterm delivery. Specifically, for every unit increase in IL-18, women were on average 1% less likely to have a preterm delivery (OR: 0.99, CI: 0.98-0.99, p=0.04). On the other hand, IL-10 was not significantly associated with preterm delivery.
Mid-trimester IL-1β concentrations are positively associated with preterm delivery. Therefore, IL-1β, determined on the occasion of mid-trimester amniocentesis could possibly serve as a marker of preterm delivery. In contrast, IL-10 and IL-18 concentrations are not elevated in mid-trimester amniotic fluid and probably cannot serve this purpose.
大量证据表明，慢性羊膜内炎症与早产的病因有关。本研究的目的是确定在接受中期羊膜穿刺术的女性中，羊水中的白细胞介素-1β（IL-1β）、白细胞介素-10（IL-10）和白细胞介素-18（IL-18）浓度是否可以识别那些有早产或早产胎膜早破风险的人。
进行了一项病例对照研究，以比较在足月或早产时分娩的女性的中期羊水中的 IL-1β、IL-10 和 IL-18 浓度。在纳入研究的 362 名女性中，有 38 名出现早产。38 名具有足月分娩的女性，按年龄和孕周匹配作为对照。排除了胎儿染色体异常或主要异常的女性。通过 ELISA 测定 IL-1β、IL-10 和 IL-18 浓度。在统计分析中应用了条件逻辑回归。
发现 IL-1β 与早产呈正相关且具有统计学意义。具体而言，IL-1β 每增加一个单位，女性平均早产的可能性增加 7.2 倍（OR：7.2，95%CI：1.94-26.77，p=0.003）。IL-18 水平以及性别与早产显著相关。具体而言，IL-18 每增加一个单位，女性平均早产的可能性降低 1%（OR：0.99，95%CI：0.98-0.99，p=0.04）。另一方面，IL-10 与早产无显著相关性。
中期 IL-1β 浓度与早产呈正相关。因此，在中期羊膜穿刺术时测定的 IL-1β 可能可作为早产的标志物。相比之下，IL-10 和 IL-18 浓度在中期羊水中并未升高，可能无法用于此目的。
