Cantonal Institute of Microbiology, Bellinzona, Switzerland.
PLoS One. 2011 Jan 25;6(1):e16424. doi: 10.1371/journal.pone.0016424.
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) allows rapid and reliable identification of microorganisms, particularly clinically important pathogens.
METHODOLOGY/PRINCIPAL FINDINGS: We compared the identification efficiency of MALDI-TOF MS with that of Phoenix®, API® and 16S ribosomal DNA sequence analysis on 1,019 strains obtained from routine diagnostics. Further, we determined the agreement of MALDI-TOF MS identifications as compared to 16S gene sequencing for additional 545 strains belonging to species of Enterococcus, Gardnerella, Staphylococcus, and Streptococcus. For 94.7% of the isolates MALDI-TOF MS results were identical with those obtained with conventional systems. 16S sequencing confirmed MALDI-TOF MS identification in 63% of the discordant results. Agreement of identification of Gardnerella, Enterococcus, Streptococcus and Staphylococcus species between MALDI-TOF MS and traditional method was high (Crohn's kappa values: 0.9 to 0.93).
CONCLUSIONS/SIGNIFICANCE: MALDI-TOF MS represents a rapid, reliable and cost-effective identification technique for clinically relevant bacteria.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）可快速可靠地鉴定微生物，尤其是临床重要病原体。
方法/主要发现：我们比较了 MALDI-TOF MS 与 Phoenix®、API®和 16S 核糖体 DNA 序列分析在 1019 株常规诊断获得的菌株中的鉴定效率。此外，我们还确定了 MALDI-TOF MS 鉴定与针对肠球菌、加德纳菌、葡萄球菌和链球菌属的另外 545 株菌株的 16S 基因测序的一致性。对于 94.7%的分离株，MALDI-TOF MS 结果与传统系统获得的结果完全一致。16S 测序在 63%的不一致结果中证实了 MALDI-TOF MS 的鉴定。MALDI-TOF MS 与传统方法鉴定加德纳菌、肠球菌、链球菌和葡萄球菌的一致性较高（克罗恩氏kappa 值：0.9 至 0.93）。
结论/意义：MALDI-TOF MS 是一种快速、可靠且具有成本效益的临床相关细菌鉴定技术。
