正常及尿毒症兔体内促红细胞生成素的代谢

The metabolism of erythropoietin in the normal and uraemic rabbit.

作者信息

Brown J H, Lappin T R, Elder G E, Bridges J M, McGeown M G

机构信息

Renal Unit, Belfast City Hospital, Northern Ireland.

出版信息

Nephrol Dial Transplant. 1990;5(10):855-9. doi: 10.1093/ndt/5.10.855.

DOI:10.1093/ndt/5.10.855

PMID:2128380

Abstract

The metabolism of recombinant human erythropoietin (rHuEpo) labelled with 125I has been investigated in five normal and nine 5/6-nephrectomised rabbits. The plasma erythropoietin half-life was significantly prolonged at 5.1 +/- 1.2 h (mean +/- SD) in the 5/6-nephrectomised rabbits, compared to 3.0 +/- 0.4 h in sham-operated controls (P less than 0.001). The disappearance of 125I-labelled rHuEpo is biphasic. Examination of serum by fast protein liquid chromatography (FPLC) following administration of 125I-labelled rHuEpo by FPLC showed a single peak of radioactivity in all rabbits except two of the nephrectomised group. In serum from both of these animals a second labelled peak was found, corresponding to material of MW 200,000-250,000 D. We conclude that the 5/6-nephrectomised rabbit provides a stable model for the study of hormonal metabolism in chronic renal failure.

摘要

在5只正常兔和9只5/6肾切除兔中研究了用125I标记的重组人促红细胞生成素（rHuEpo）的代谢。与假手术对照组的3.0±0.4小时相比，5/6肾切除兔的血浆促红细胞生成素半衰期显著延长至5.1±1.2小时（平均值±标准差）（P<0.001）。125I标记的rHuEpo的消失是双相的。通过快速蛋白质液相色谱法（FPLC）给兔注射125I标记的rHuEpo后，对血清进行FPLC分析，结果显示除了两只肾切除组的兔子外，所有兔子的血清中都有一个放射性单峰。在这两只动物的血清中发现了第二个标记峰，对应于分子量为200,000-250,000 D的物质。我们得出结论，5/6肾切除兔为慢性肾衰竭激素代谢的研究提供了一个稳定的模型。