Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Mass Spectrom Rev. 2011 Nov-Dec;30(6):1256-68. doi: 10.1002/mas.20331. Epub 2011 Jan 31.
This article reviews the development of and applications for high irradiance laser ionization orthogonal time-of-flight mass spectrometry (LI-O-TOFMS). LI-O-TOFMS has solved the bottleneck problems in traditional high irradiance laser ionization mass spectrometry, which allows the instrument to acquire explicit spectra with high resolution. A buffer-gas-assisted ion source effectively reduces the kinetic energy of the ions and suppresses the multiply charged ion interference. The pulse train data acquisition technique was applied to reduce the spectrum interference from multiply charged ions and polyatomic ions according to the temporal profiles of different ion packets in the repelling region. Relatively high laser irradiance (≥10(10) W/cm(2)) is preferable for achieving uniform relative sensitivities for different elements in the samples of different matrices. LI-O-TOFMS has been used in the standardless, semiquantitative analysis of solids, which is proved to be a fast and convenient technique for solid sample analysis. By increasing the laser irradiance and reducing the buffer gas pressure, the determination of nonmetallic elements in solids can also be achieved without losing spectral explicity. Recent applications, such as elemental analysis of a single egg cell and acquiring elemental, fragmental, and molecular information of chemicals, were given to demonstrate the potential of the new technique. All of these results reveal that LI-O-TOFMS is an advanced tool in the elemental analysis of solids in terms of modern mass spectrometry.
本文综述了高辐照度激光电离正交飞行时间质谱（LI-O-TOFMS）的发展和应用。LI-O-TOFMS 解决了传统高辐照度激光电离质谱中的瓶颈问题，使仪器能够获得具有高分辨率的明确光谱。缓冲气体辅助离子源有效地降低了离子的动能，并抑制了多电荷离子的干扰。脉冲串数据采集技术根据排斥区中不同离子包的时间分布来减少来自多电荷离子和多原子离子的光谱干扰。相对较高的激光辐照度（≥10(10) W/cm(2)）更有利于实现不同基质样品中不同元素的相对灵敏度均匀性。LI-O-TOFMS 已用于无标准、半定量分析固体，被证明是一种快速方便的固体样品分析技术。通过增加激光辐照度和降低缓冲气体压力，即使在不损失光谱清晰度的情况下，也可以实现对固体中非金属元素的测定。最近的应用，如单细胞的元素分析以及获取化学物质的元素、碎片和分子信息，都证明了这项新技术的潜力。所有这些结果都表明，LI-O-TOFMS 是现代质谱学中固体元素分析的一种先进工具。
