Center for Experimental Therapeutics and Reperfusion Injury, Harvard Institutes of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.
Inflammation. 2012 Feb;35(1):98-113. doi: 10.1007/s10753-011-9294-8.
Temporal-metabolomic studies of local mediators during inflammation and its resolution uncovered novel pathways and mediators, e.g., lipoxins, resolvins, and protectins that stimulate key resolution responses. Since these studies were carried out with isolated human cells and in animal models, it is important to determine in humans whether temporal profiles between pro-inflammatory mediators and pro-resolving mediators are demonstrable in vivo. To this end, we examined patients undergoing abdominal aortic aneurysm (AAA) surgery. Profiles of mediators including eicosanoids were assessed in addition to pro-resolving mediators. The results demonstrate temporal relationships for local-acting peptides (e.g., VEGF, IL-10, TGF(β)) and lipid mediators (leukotrienes and resolvins). In addition, profiles obtained for AAA patients divided into two groups based on their temporal profile: one group consistent with a pro-inflammatory and another with a resolving profile. Together, these translational metabolomic profiles demonstrate for the first time the temporal relationships between local mediators in humans relevant in inflammation resolution.
在炎症及其消退过程中的时间代谢组学研究揭示了新的途径和介质，例如脂氧素、消退素和保护素，它们刺激关键的消退反应。由于这些研究是在分离的人类细胞和动物模型中进行的，因此重要的是要确定在人类中，促炎介质和促消退介质之间的时间曲线是否在体内表现出来。为此，我们检查了接受腹主动脉瘤 (AAA) 手术的患者。除了促消退介质外，还评估了包括类二十烷酸在内的介质的谱。结果表明，局部作用肽（例如，VEGF、IL-10、TGF(β)）和脂质介质（白三烯和消退素）之间存在时间关系。此外，根据时间曲线将 AAA 患者分为两组：一组与促炎谱一致，另一组与消退谱一致。总之，这些转化代谢组学谱首次证明了人类体内与炎症消退相关的局部介质之间的时间关系。
