Craniofacial and Skeletal Diseases Branch, National Institutes of Dental and Craniofacial Research, Bethesda, Maryland 20892, USA.
Stem Cells Dev. 2012 Jan;21(1):152-7. doi: 10.1089/scd.2010.0593. Epub 2011 Mar 17.
Skin samples taken from 6 female patients receiving allogeneic bone marrow transplants (BMT) from male siblings (n=5) or from unrelated human leukocyte antigen (HLA)-matched male donor (n=1) due to hematological malignancies were studied for the presence of donor cells. One nontransplanted male and 1 female control that received female BM were used as further controls of the technique. Skin biopsies were taken from the scalp and the back from each patient 12-16 years after the successful BMT. We have found donor chimerism in all of the 6 patients in both of their biopsies. Using single and double immunostainings in combination with Y chromosome hybridization, we observed that there are cytokeratin-expressing donor-derived cells in the epidermis of all the 6 patients, the numbers being slightly higher in the scalp (0.37%-1.78%) than in the back (0.32%-1.08%) biopsies. The indication for BMT, and the age of the patient did not seem to have any effect on the numbers found. A few of the double-labeled cells also stained for Ki67, a marker of cellular proliferation, suggesting that the engrafted cells were able to further divide in the epidermis. In 2 patients we observed patches of donor keratinocytes within the epidermis, suggesting a clonal origin. We conclude that in agreement with some and in contrast to other published studies, BM-derived circulating cells are able to engraft in the human skin and to further proliferate there and thus contribute to tissue renewal. These data raise the possibility to use BM cells in regenerative medicine to help in extended injuries, large surface burns, or lack of skin due to other reasons.
对 6 名因血液系统恶性肿瘤而接受来自男性兄弟姐妹（n=5）或与无关人类白细胞抗原（HLA）匹配的男性供体（n=1）的异基因骨髓移植（BMT）的女性患者的皮肤样本进行了研究，以检测供体细胞的存在。1 名未接受移植的男性和 1 名接受女性 BM 的女性对照进一步作为该技术的对照。在成功接受 BMT 后 12-16 年，从每位患者的头皮和背部采集皮肤活检。我们发现 6 名患者的两种活检均存在供体嵌合体。通过使用单免疫染色和双免疫染色结合 Y 染色体杂交，我们观察到 6 名患者的表皮中均存在表达细胞角蛋白的供体衍生细胞，头皮（0.37%-1.78%）中的数量略高于背部（0.32%-1.08%）。BMT 的适应证和患者年龄似乎对发现的数量没有影响。少数双标记细胞也对细胞增殖标志物 Ki67 染色，表明植入细胞能够在表皮中进一步分裂。在 2 名患者中，我们观察到表皮内存在供体角质形成细胞斑块，提示克隆起源。我们得出结论，与一些已发表的研究一致，与其他一些已发表的研究相反，BM 来源的循环细胞能够在人体皮肤中植入，并在那里进一步增殖，从而有助于组织更新。这些数据提出了在再生医学中使用 BM 细胞来帮助治疗广泛的损伤、大面积烧伤或因其他原因导致的皮肤缺失的可能性。
