Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
Genes Dev. 2011 Feb 1;25(3):214-9. doi: 10.1101/gad.1993611.
In Schizosaccharomyces pombe, the RNAi pathway is required for the formation of pericentric heterochromatin, proper chromosome segregation, and repression of pericentric meiotic recombination. Here we demonstrate that, when the activity of the histone H3 Lys 14 (H3K14) acetyltransferase Mst2 is eliminated, the RNAi machinery is no longer required for pericentric heterochromatin functions. We further reveal that reducing RNA polymerase II recruitment to pericentric regions is essential for maintaining heterochromatin in the absence of RNAi.
在裂殖酵母中，RNAi 途径对于着丝粒异染色质的形成、正确的染色体分离以及着丝粒减数重组的抑制是必需的。在这里，我们证明了当组蛋白 H3 赖氨酸 14（H3K14）乙酰转移酶 Mst2 的活性被消除时，RNAi 机制不再是着丝粒异染色质功能所必需的。我们进一步揭示了减少 RNA 聚合酶 II 向着丝粒区域的募集对于在没有 RNAi 的情况下维持异染色质是必不可少的。
