CMUA, Department of Electrical and Electronics Engineering, Universidad de los Andes, Bogota, Colombia.
FEMS Microbiol Lett. 2011 May;318(1):27-34. doi: 10.1111/j.1574-6968.2011.02234.x. Epub 2011 Mar 1.
In this paper, we studied the laccase production and the growth morphology of different white-rot fungi, i.e. Pleurotus ostreatus, Trametes pubescens, Cerrena unicolor and Trametes versicolor, cultured under semi-solid-state fermentation conditions using wheat bran flakes as a natural low-cost support substrate. Trametes versicolor exhibited the highest laccase activity per gram of total dry matter, followed by P. ostreatus (63.5 and 58.2Ug(-1) , respectively). In addition, they showed a time profile of laccase production that was quite similar. Growth morphology was studied using environmental microscopic images and analyzed by discrete Fourier transformation-based software to determine the mean diameter of the hyphae, the number of hypha layers and the global micromorphology. The four strains exhibited different micromorphologies of growth. Pleurotus ostreatus presented narrow hyphae, which formed many thick clumps, T. pubescens and T. versicolor showed clumps of different sizes and C. unicolor showed thick hyphae that formed larger clumps, but in less amounts.
在本文中，我们研究了不同白腐真菌（即平菇、绒栓菌、红栓菌和彩绒革盖菌）在半固态发酵条件下利用麸皮作为天然低成本支持底物时的产漆酶和生长形态。彩绒革盖菌每克总干物质的漆酶活性最高，其次是平菇（分别为 63.5 和 58.2Ug(-1)）。此外，它们表现出相当相似的产漆酶时间曲线。使用环境显微镜图像研究生长形态，并通过基于离散傅里叶变换的软件进行分析，以确定菌丝的平均直径、菌丝层数和全局微观形态。四种菌株表现出不同的生长微观形态。平菇呈现出狭窄的菌丝，形成许多厚厚的团块，绒栓菌和彩绒革盖菌显示出不同大小的团块，而红栓菌则显示出形成较大团块的厚菌丝，但数量较少。
