First Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary.
Clin Immunol. 2011 Apr;139(1):48-56. doi: 10.1016/j.clim.2010.12.018. Epub 2011 Feb 2.
The purpose of this study was to determine whether the proportion of galectin-1-expressing peripheral blood T and NK cells is altered in normal pregnancy and preeclampsia (PE). We also examined whether circulating levels of galectin-1 and anti-galectin-1 autoantibodies are affected in PE. Seventy preeclamptic patients, 75 healthy pregnant and 21 healthy non-pregnant women were involved in this study. Serum galectin-1 and anti-galectin-1 autoantibody levels were measured by ELISA. Intracellular galectin-1 expression of lymphocytes was determined with flow cytometry. Serum galectin-1 and anti-galectin-1 IgG levels did not differ significantly between the healthy pregnant and the PE group. In healthy pregnant women, significantly higher percentage of T and NK cells expressed gal-1 in their cytoplasma than in healthy non-pregnant women. However, the proportion of galectin-1-expressing peripheral blood T and NK cells was markedly decreased in PE compared to normal pregnancy, which might contribute to the activation of innate and acquired immune cells.
本研究旨在确定在正常妊娠和子痫前期（PE）中，外周血 T 和 NK 细胞中表达 galectin-1 的比例是否发生改变。我们还研究了循环 galectin-1 水平和抗 galectin-1 自身抗体在 PE 中是否受到影响。本研究共纳入 70 例子痫前期患者、75 例健康孕妇和 21 例健康非孕妇。通过 ELISA 测定血清 galectin-1 和抗 galectin-1 自身抗体水平。通过流式细胞术测定淋巴细胞内 galectin-1 的表达。健康孕妇与 PE 组之间血清 galectin-1 和抗 galectin-1 IgG 水平无显著差异。在健康孕妇中，外周血 T 和 NK 细胞的细胞质中表达 gal-1 的百分比明显高于健康非孕妇。然而，与正常妊娠相比，PE 中外周血 T 和 NK 细胞中表达 galectin-1 的比例明显降低，这可能导致先天和获得性免疫细胞的激活。
