韩国西南部恙虫病东方体 56 kDa 蛋白基因的系统进化分析。
Phylogenetic analysis of the 56 kDa protein genes of Orientia tsutsugamushi in Southwest Area of Korea.
机构信息
Division of Infectious Diseases, Departments of Internal Medicine, School of Medicine, Chosun University, Gwangju, Korea.
出版信息
Am J Trop Med Hyg. 2011 Feb;84(2):250-4. doi: 10.4269/ajtmh.2011.09-0601.
Abstract
This study was conducted to determine which genotypes were present in southwestern Korea. Nested polymerase chain reaction (PCR) and DNA sequence analysis targeting the Orientia tsutsugamushi-specific 56-kDa protein gene was performed with samples of blood and eschar. Of the 69 PCR-positive samples, 61 clustered with the Boryong previously isolated in Korea. CUH 4-6 had sequence homology of 100% with Kato, and CUH 4-3 had homology of 99.8% with Kato and formed the Kato cluster. CUH 4-57, CUH 4-31, CUH 4-142, and CUH 4-324 formed a Kawasaki cluster. CUH 4-271 had sequence homology of 100% with Jecheon and formed a Karp cluster. CUH 4-117 had homology of 99.8% with Neimeng-65, and Gilliam cluster. The most common genotype of O. tsutsugamushi in the southwestern part of Korea is the Boryong genotype. We also identified O. tsutsugamushi of the Kato, Neimeng-65 and Kawasaki genotypes, which had not been identified before in Korea.
摘要
本研究旨在确定韩国西南部存在哪些基因型。使用血液和焦痂样本，通过巢式聚合酶链反应（PCR）和针对恙虫东方体特异性 56kDa 蛋白基因的 DNA 序列分析进行了研究。在 69 个 PCR 阳性样本中，有 61 个与韩国先前分离的 Boryong 聚类。CUH 4-6 与 Kato 的序列同源性为 100%，而 CUH 4-3 与 Kato 的同源性为 99.8%，并形成了 Kato 聚类。CUH 4-57、CUH 4-31、CUH 4-142 和 CUH 4-324 形成了 Kawasaki 聚类。CUH 4-271 与 Jecheon 的序列同源性为 100%，形成了 Karp 聚类。CUH 4-117 与 Neimeng-65 和 Gilliam 聚类的同源性为 99.8%。韩国西南部最常见的恙虫东方体基因型是 Boryong 基因型。我们还鉴定了以前在韩国未鉴定到的 Kato、Neimeng-65 和 Kawasaki 基因型的恙虫东方体。
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