一种用于检测四种土壤传播性蠕虫的五重实时聚合酶链反应检测方法。
A pentaplex real-time polymerase chain reaction assay for detection of four species of soil-transmitted helminths.
机构信息
Institute for Research in Molecular Medicine, Universiti Sains Malaysia (USM), Penang, Malaysia.
出版信息
Am J Trop Med Hyg. 2011 Feb;84(2):338-43. doi: 10.4269/ajtmh.2011.10-0499.
Abstract
Soil-transmitted helminth infections remain a major public health burden in low- and middle-income countries. The traditional diagnosis by microscopic examination of fecal samples is insensitive and time-consuming. In this study, a pentaplex real-time polymerase chain reaction (PCR) was evaluated for the simultaneous detection of Ancylostoma, Necator americanus, Ascaris lumbricoides, and Strongyloides stercoralis. The results were compared with those obtained by conventional parasitological diagnostic methods. Real-time PCR was positive in 48 of 77 samples (62.3%) and microscopic examination was positive in six samples (7.8%) only (P < 0.05). In conclusion, the real-time PCR assay described in this study provides a specific and sensitive diagnostic tool for the detection of these four helminth species in epidemiological studies and monitoring of treatment programs.
摘要
土壤传播性蠕虫感染仍然是中低收入国家的主要公共卫生负担。传统的粪便样本显微镜检查诊断方法不敏感且耗时。在这项研究中，评估了五重实时聚合酶链反应（PCR）同时检测钩虫、美洲板口线虫、蛔虫和粪类圆线虫。结果与传统寄生虫学诊断方法进行了比较。实时 PCR 在 77 个样本中的 48 个（62.3%）呈阳性，而显微镜检查仅在 6 个样本（7.8%）中呈阳性（P < 0.05）。总之，本研究中描述的实时 PCR 检测方法为在流行病学研究和治疗方案监测中检测这四种蠕虫提供了一种特异性和敏感性的诊断工具。
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