Department of Human Morphology, Neurosurgical Unit, University of Insubria, Varese, Italy.
J Neurosurg. 2011 Jun;114(6):1723-30. doi: 10.3171/2010.12.JNS101732. Epub 2011 Feb 4.
Human dura mater is the most external meningeal sheet surrounding the CNS. It provides an efficient protection to intracranial structures and represents the most important site for CSF turnover. Its intrinsic architecture is made up of fibrous tissue including collagenic and elastic fibers that guarantee the maintenance of its biophysical features. The recent technical advances in the repair of dural defects have allowed for the creation of many synthetic and biological grafts. However, no detailed studies on the 3D microscopic disposition of collagenic fibers in dura mater are available. The authors report on the collagenic 3D architecture of normal dura mater highlighting the orientation, disposition in 3 dimensions, and shape of the collagen fibers with respect to the observed layer.
Thirty-two dura mater specimens were collected during cranial decompressive surgical procedures, fixed in 2.5% Karnovsky solution, and digested in 1 N NaOH solution. After a routine procedure, the specimens were observed using a scanning electron microscope.
The authors distinguished the following 5 layers in the fibrous dura mater of varying thicknesses, orientation, and structures: bone surface, external median, vascular, internal median, and arachnoid layers.
The description of the ultrastructural 3D organization of the different layers of dura mater will give us more information for the creation of synthetic grafts that are as similar as possible to normal dura mater. This description will be also related to the study of the neoplastic invasion.
硬脑膜是环绕中枢神经系统的最外层脑膜。它为颅内结构提供了有效的保护，是脑脊液周转的最重要部位。其固有结构由纤维组织组成，包括胶原纤维和弹性纤维，保证了其生物物理特性的维持。最近在硬脑膜缺损修复方面的技术进步已经允许创建许多合成和生物移植物。然而，关于硬脑膜中胶原纤维的 3D 微观排列，还没有详细的研究。作者报告了正常硬脑膜的胶原 3D 结构，重点介绍了胶原纤维相对于观察到的层的取向、3 维排列和形状。
在颅减压手术过程中收集了 32 个硬脑膜标本，用 2.5%的 Karnovsky 溶液固定，并用 1N NaOH 溶液消化。经过常规处理后，使用扫描电子显微镜观察标本。
作者在不同厚度、取向和结构的纤维硬脑膜中区分出以下 5 层：骨表面、外部正中、血管、内部正中、和蛛网膜层。
描述硬脑膜不同层的超微结构 3D 组织将为我们提供更多信息，以便创建尽可能类似于正常硬脑膜的合成移植物。这种描述还将与肿瘤侵袭的研究相关。
