Sun Y-Z, Yang H-L, Ma R-L, Song K, Lin W-Y
The Key Laboratory of Science and Technology for Aquaculture and Food Safety of Fujian Province University, Fisheries College, Jimei University, Xiamen, China The Key Laboratory for Feed Quality Testing and Safety Evaluation of Xiamen, Fisheries College, Jimei University, Xiamen, China.
J Appl Microbiol. 2011 Apr;110(4):1093-103. doi: 10.1111/j.1365-2672.2011.04967.x. Epub 2011 Feb 23.
To evaluate the diversity of dominant autochthonous microbiota along the digestive tract of juvenile Epinephelus coioides following the dietary administration of probiotic Bacillus pumilus for 60 days.
Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) with subsequently sequencing analysis was used to assess the gut microbiota. Generally similar DGGE patterns were observed in the foregut, midgut and hindgut of E. coioides, while the similarity dendrogram clearly revealed three different clusters depending on the three compartments of the GI tract. Dietary administration of B. pumilus stimulated its colonization in each compartment of the digestive tract. Samples collected from the probiotic group and the control group showed similar DGGE patterns, and no significant difference in the total number of bands and the Shannon index were detected between the probiotic group and the control group, suggested that B. pumilus exert no significant effect on the gut microbiota. However, various potentially beneficial bacteria, such as uncultured Bacillus sp. clone QJNY94-like, Nitratireductor sp. YCSC5-like, Methylobacterium hispanicum-like and Microbacterium sp. YACS1-like bacteria were stimulated by probiotic B. pumilus, while the potential harmful Staphylococcus saprophyticus-like bacterium was depressed.
Autochthonous gut microbiota of E. coioides was modulated to some degree, not significant, by probiotic B. pumilus, various potentially beneficial bacteria were selectively stimulated, while one potential harmful species was depressed.
This work represents the first report that dietary administration of probiotic B. pumilus modulated the gut microbiota of E. coioides. These findings broaden our understanding of probiotic effects at the gut level, which is helpful in understanding the mechanisms that underpin host benefits.
评估在饲料中添加短小芽孢杆菌60天对斜带石斑鱼幼鱼消化道内优势本土微生物群多样性的影响。
采用聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）并结合测序分析来评估肠道微生物群。斜带石斑鱼的前肠、中肠和后肠观察到的DGGE图谱总体相似，而相似性树状图根据胃肠道的三个部分清晰地显示出三个不同的聚类。饲料中添加短小芽孢杆菌促进了其在消化道各部分的定殖。益生菌组和对照组采集的样本显示出相似的DGGE图谱，益生菌组和对照组之间在条带总数和香农指数上没有显著差异，表明短小芽孢杆菌对肠道微生物群没有显著影响。然而，各种潜在有益细菌，如未培养的芽孢杆菌属克隆QJNY94样、硝酸盐还原菌属YCSC5样、西班牙甲基杆菌样和微小杆菌属YACS1样细菌受到益生菌短小芽孢杆菌的刺激，而潜在有害的腐生葡萄球菌样细菌受到抑制。
短小芽孢杆菌对斜带石斑鱼的本土肠道微生物群有一定程度的调节作用，但不显著，它选择性地刺激了各种潜在有益细菌，同时抑制了一种潜在有害细菌。
这项工作首次报道了饲料中添加益生菌短小芽孢杆菌对斜带石斑鱼肠道微生物群的调节作用。这些发现拓宽了我们对益生菌在肠道水平作用的理解，有助于理解宿主获益的潜在机制。
