Key Laboratory of Textile Science and Technology Ministry of Education, Donghua University, Shanghai 201620, P. R. China.
J Biomater Sci Polym Ed. 2012;23(1-4):497-508. doi: 10.1163/092050610X552771. Epub 2011 Jan 28.
As an excellent biocompatible and biodegradable protein polymer, silk fibroin (SF) has found wide applications, particularly serving as therapeutic agent for tissue-engineering applications, on which both post-spin treatment and sterilization processing are crucial to drug-loaded matrices. To find a safe, effective and appropriate post-spin treatment and sterilization approach for drug-loaded biomaterial matrices is one of the major problems in the field of tissue engineering at present. In this work, a simple, safe and effective approach skillfully integrating post-spin treatment with sterilization processing was developed to drug-loaded SF nanofibrous matrices. Electrospun SF nanofibrous matrices from its aqueous solution were post-treated with 75% ethanol vapor. (13)C-NMR and WAXD analysis demonstrated that such post-spin treatment rendered the structure of SF nanofibrous matrices transform from the silk I form to the silk II form. Furthermore, biological assays suggested that as-treated SF nanofibrous matrices significantly promoted the development of murine connective tissue fibroblasts. Skillfully integrated with novel sterilization processing, 75% ethanol vapor treatment could be a potential approach to designing and fabricating diverse drug-loaded SF nanofibrous matrices serving as therapeutic agents for tissue-engineering applications in that it can effectively protect the drug from losing compared with traditional post-spin treatment and sterilization processing.
作为一种优秀的生物相容性和可生物降解的蛋白质聚合物，丝素（SF）已经得到了广泛的应用，特别是作为组织工程应用的治疗剂，其纺丝后处理和灭菌处理对于载药基质都至关重要。寻找一种安全、有效和合适的载药生物材料基质的纺丝后处理和灭菌方法是当前组织工程领域的主要问题之一。在这项工作中，开发了一种简单、安全、有效的方法，巧妙地将纺丝后处理与灭菌处理结合起来，用于载药 SF 纳米纤维基质。从丝素水溶液中电纺的 SF 纳米纤维基质用 75%乙醇蒸汽进行后处理。（13）C-NMR 和 WAXD 分析表明，这种纺丝后处理使 SF 纳米纤维基质的结构从丝素 I 型转变为丝素 II 型。此外，生物分析表明，经处理的 SF 纳米纤维基质显著促进了鼠结缔组织成纤维细胞的发育。巧妙地与新型灭菌处理相结合，75%乙醇蒸汽处理可能是设计和制造各种用作组织工程应用治疗剂的载药 SF 纳米纤维基质的潜在方法，因为与传统的纺丝后处理和灭菌处理相比，它可以有效地保护药物不流失。
