Hashimoto Tomoko, Kojima Katsura, Tamada Yasushi
Silk Materials Research Unit, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan.
Faculty of Human Life and Environment, Nara Women's University, Kitauoya-nishimachi, Nara 6308506, Japan.
Molecules. 2020 Apr 22;25(8):1939. doi: 10.3390/molecules25081939.
Silk fibroin (SF), which offers the benefits of biosafety, biocompatibility, and mechanical strength, has potential for use as a good biomedical material, especially in the tissue engineering field. This study investigated the use of SF biomaterials as a wound dressing compared to commercially available collagen materials. After human fibroblasts (WI-38) were cultured on both films and sponges, their cell motilities and gene expressions related to wound repair and tissue reconstruction were evaluated. Compared to the collagen film (Col film), the SF film induced higher cell motility; higher expressions of genes were observed on the SF film. Extracellular matrix production-related genes were up-regulated in WI-38 fibroblasts cultured on the SF sponges. These results suggest that SF-based biomaterials can accelerate wound healing and tissue reconstruction. They can be useful biomaterials for functional wound dressings.
丝素蛋白（SF）具有生物安全性、生物相容性和机械强度等优点，有潜力作为一种良好的生物医学材料，尤其是在组织工程领域。本研究调查了与市售胶原蛋白材料相比，SF生物材料作为伤口敷料的用途。在人成纤维细胞（WI-38）在薄膜和海绵上培养后，评估了它们与伤口修复和组织重建相关的细胞运动性和基因表达。与胶原蛋白薄膜（Col薄膜）相比，SF薄膜诱导了更高的细胞运动性；在SF薄膜上观察到更高的基因表达。在SF海绵上培养的WI-38成纤维细胞中，与细胞外基质产生相关的基因上调。这些结果表明，基于SF的生物材料可以加速伤口愈合和组织重建。它们可以成为用于功能性伤口敷料的有用生物材料。
