Renauld J C, Goethals A, Houssiau F, Van Roost E, Van Snick J
Unit of Experimental Medicine, Université Catholique de Louvain, Belgium.
Cytokine. 1990 Jan;2(1):9-12. doi: 10.1016/1043-4666(90)90037-t.
A cDNA encoding the human homolog of mouse T-cell and mast cell growth factor P40 was derived from peripheral blood mononuclear cells (PBMC) stimulated with phytohemagglutinin and phorbol myristate acetate. Sequence analysis of the cDNA predicted a precursor protein of 144 amino acids including a signal peptide of 18 residues, a structure identical with that of mouse P40. The homology between the mouse and human proteins is 55% with a perfect conservation of the 10 cysteine residues present in the mature polypeptide. Expression of the cDNA for human P40 in a baculovirus vector yielded a protein capable of enhancing in vitro survival of human T cell lines.
通过用植物血凝素和佛波醇肉豆蔻酸酯乙酸盐刺激外周血单核细胞（PBMC），获得了编码小鼠T细胞和肥大细胞生长因子P40的人类同源物的cDNA。对该cDNA的序列分析预测其前体蛋白有144个氨基酸，包括一个18个残基的信号肽，其结构与小鼠P40相同。小鼠和人类蛋白质之间的同源性为55%，成熟多肽中存在的10个半胱氨酸残基完全保守。在杆状病毒载体中表达人类P40的cDNA产生了一种能够增强人类T细胞系体外存活率的蛋白质。
