含高浓度 DNA/PEI 聚集体的透明质酸和纤维蛋白水凝胶用于局部基因递送。
Hyaluronic acid and fibrin hydrogels with concentrated DNA/PEI polyplexes for local gene delivery.
机构信息
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, BH5531, Los Angeles, CA 90095, USA.
出版信息
J Control Release. 2011 Aug 10;153(3):255-61. doi: 10.1016/j.jconrel.2011.01.028. Epub 2011 Feb 2.
Abstract
Local delivery of DNA through a hydrogel scaffold would increase the applicability of gene therapy in tissue regeneration and cancer therapy. However, the delivery of DNA/cationic polymer nanoparticles (polyplexes) using hydrogels is challenging due to the aggregation and inactivation of polyplexes during their incorporation into hydrogel scaffolds. We developed a novel process (termed caged nanoparticle encapsulation or CnE) to load concentrated and unaggregated non-viral gene delivery nanoparticles into various hydrogels. Previously, we showed that PEG hydrogels loaded with DNA/PEI polyplexes through this process were able to deliver genes both in vitro and in vivo. In this study, we found that hyaluronic acid and fibrin hydrogels with concentrated and unaggregated polyplexes loaded through CnE were able to deliver genes in vivo as well, demonstrating the universality of the process.
摘要
通过水凝胶支架进行局部 DNA 递送将增加基因治疗在组织再生和癌症治疗中的适用性。然而，由于 DNA/阳离子聚合物纳米颗粒（聚合物）在掺入水凝胶支架过程中的聚集和失活，使用水凝胶进行 DNA 递送具有挑战性。我们开发了一种新的方法（称为笼状纳米颗粒包封或 CnE），将浓缩且未聚集的非病毒基因传递纳米颗粒加载到各种水凝胶中。此前，我们表明，通过该方法将 DNA/PEI 聚合物纳米颗粒载入聚乙二醇水凝胶中，无论是在体外还是体内，都能够递送基因。在这项研究中，我们发现，通过 CnE 载入浓缩且未聚集的聚合物纳米颗粒的透明质酸和纤维蛋白水凝胶也能够在体内递送基因，证明了该方法的普遍性。
相似文献
1
Hyaluronic acid and fibrin hydrogels with concentrated DNA/PEI polyplexes for local gene delivery.含高浓度 DNA/PEI 聚集体的透明质酸和纤维蛋白水凝胶用于局部基因递送。
J Control Release. 2011 Aug 10;153(3):255-61. doi: 10.1016/j.jconrel.2011.01.028. Epub 2011 Feb 2.
2
Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation.将聚乙二醇化的低分子量聚乙烯亚胺多聚体包裹于透明质酸水凝胶中可减少聚集。
Acta Biomater. 2015 Dec;28:45-54. doi: 10.1016/j.actbio.2015.09.020. Epub 2015 Sep 21.
3
Incorporation of active DNA/cationic polymer polyplexes into hydrogel scaffolds.将活性 DNA/阳离子聚合物聚集体掺入水凝胶支架中。
Biomaterials. 2010 Dec;31(34):9106-16. doi: 10.1016/j.biomaterials.2010.08.016. Epub 2010 Sep 6.
4
Design and characterization of microporous hyaluronic acid hydrogels for in vitro gene transfer to mMSCs.设计并表征用于体外向间充质干细胞转染基因的多孔透明质酸水凝胶。
Acta Biomater. 2012 Nov;8(11):3921-31. doi: 10.1016/j.actbio.2012.07.014. Epub 2012 Jul 20.
5
DNA delivery from matrix metalloproteinase degradable poly(ethylene glycol) hydrogels to mouse cloned mesenchymal stem cells.从基质金属蛋白酶可降解的聚乙二醇水凝胶向小鼠克隆间充质干细胞递送DNA。
Biomaterials. 2009 Jan;30(2):254-65. doi: 10.1016/j.biomaterials.2008.09.027. Epub 2008 Oct 5.
6
Porous hyaluronic acid hydrogels for localized nonviral DNA delivery in a diabetic wound healing model.用于糖尿病伤口愈合模型中局部非病毒DNA递送的多孔透明质酸水凝胶
Adv Healthc Mater. 2015 May;4(7):1084-91. doi: 10.1002/adhm.201400783. Epub 2015 Feb 18.
7
Non-viral vector delivery from PEG-hyaluronic acid hydrogels.来自聚乙二醇-透明质酸水凝胶的非病毒载体递送
J Control Release. 2007 Jul 31;120(3):233-41. doi: 10.1016/j.jconrel.2007.04.015. Epub 2007 May 1.
8
Junk DNA enhances pEI-based non-viral gene delivery.垃圾 DNA 增强基于 pEI 的非病毒基因传递。
Int J Pharm. 2010 May 5;390(1):76-83. doi: 10.1016/j.ijpharm.2009.08.032. Epub 2009 Aug 29.
9
Design of PEI-conjugated bio-reducible polymer for efficient gene delivery.PEI 接枝生物还原聚合物的设计用于高效基因传递。
Int J Pharm. 2018 Jul 10;545(1-2):295-305. doi: 10.1016/j.ijpharm.2018.04.051. Epub 2018 Apr 23.
10
Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration.可注射和可调的透明质酸水凝胶，用于释放趋化因子和血管生成生长因子的牙髓再生。
Acta Biomater. 2018 Sep 1;77:155-171. doi: 10.1016/j.actbio.2018.07.035. Epub 2018 Jul 18.
引用本文的文献
1
Insights into Translational and Biomedical Applications of Hydrogels as Versatile Drug Delivery Systems.水凝胶作为多功能药物输送系统的转化和生物医学应用的新见解。
AAPS PharmSciTech. 2024 Jan 22;25(1):17. doi: 10.1208/s12249-024-02731-y.
2
Tissue-Engineered Nanomaterials Play Diverse Roles in Bone Injury Repair.组织工程纳米材料在骨损伤修复中发挥着多种作用。
Nanomaterials (Basel). 2023 Apr 24;13(9):1449. doi: 10.3390/nano13091449.
3
Matrices Activated with Messenger RNA.用信使核糖核酸激活的基质。
J Funct Biomater. 2023 Jan 15;14(1):48. doi: 10.3390/jfb14010048.
4
Tuning Peptide-Based Hydrogels: Co-Assembly with Composites Driving the Highway to Technological Applications.基于肽的水凝胶的调谐：与复合材料的共组装推动技术应用的高速公路。
Int J Mol Sci. 2022 Dec 22;24(1):186. doi: 10.3390/ijms24010186.
5
In vivo restoration of dystrophin expression in mdx mice using intra-muscular and intra-arterial injections of hydrogel microsphere carriers of exon skipping antisense oligonucleotides.利用肌内和动脉内注射包含外显子跳跃反义寡核苷酸的水凝胶微球载体，在 mdx 小鼠体内恢复肌营养不良蛋白的表达。
Cell Death Dis. 2022 Sep 9;13(9):779. doi: 10.1038/s41419-022-05166-0.
6
pH-responsive polymeric vesicles from branched copolymers.来自支化共聚物的pH响应性聚合物囊泡。
RSC Adv. 2019 Dec 11;9(70):41031-41037. doi: 10.1039/c9ra08703f. eCollection 2019 Dec 9.
7
Development of a fibrin-mediated gene delivery system for the treatment of cystinosis via design of experiment.通过实验设计开发用于治疗胱氨酸病的纤维蛋白介导的基因递送系统。
Sci Rep. 2022 Mar 8;12(1):3752. doi: 10.1038/s41598-022-07750-y.
8
Smart/stimuli-responsive hydrogels: Cutting-edge platforms for tissue engineering and other biomedical applications.智能/刺激响应水凝胶：用于组织工程和其他生物医学应用的前沿平台。
Mater Today Bio. 2021 Dec 9;13:100186. doi: 10.1016/j.mtbio.2021.100186. eCollection 2022 Jan.
9
Nucleic Acid Delivery from Granular Hydrogels.颗粒状水凝胶中的核酸递送。
Adv Healthc Mater. 2022 Feb;11(3):e2101867. doi: 10.1002/adhm.202101867. Epub 2021 Nov 23.
10
The biological applications of DNA nanomaterials: current challenges and future directions.DNA 纳米材料的生物学应用：当前的挑战和未来的方向。
Signal Transduct Target Ther. 2021 Oct 8;6(1):351. doi: 10.1038/s41392-021-00727-9.
本文引用的文献
1
Incorporation of active DNA/cationic polymer polyplexes into hydrogel scaffolds.将活性 DNA/阳离子聚合物聚集体掺入水凝胶支架中。
Biomaterials. 2010 Dec;31(34):9106-16. doi: 10.1016/j.biomaterials.2010.08.016. Epub 2010 Sep 6.
2
Localized and sustained delivery of silencing RNA from macroscopic biopolymer hydrogels.从宏观生物聚合物水凝胶中实现沉默RNA的局部和持续递送。
J Am Chem Soc. 2009 Jul 8;131(26):9204-6. doi: 10.1021/ja9037615.
3
Cell-controlled and spatially arrayed gene delivery from fibrin hydrogels.来自纤维蛋白水凝胶的细胞控制和空间排列的基因递送。
Biomaterials. 2009 Aug;30(22):3790-9. doi: 10.1016/j.biomaterials.2009.03.049. Epub 2009 Apr 23.
4
Fibrin-lipoplex system for controlled topical delivery of multiple genes.用于多种基因可控局部递送的纤维蛋白-脂质体复合物系统。
Biomacromolecules. 2009 Jun 8;10(6):1650-4. doi: 10.1021/bm900248n.
5
A matrix reservoir for improved control of non-viral gene delivery.一种用于改善非病毒基因递送控制的基质储库。
J Control Release. 2009 Jun 19;136(3):220-5. doi: 10.1016/j.jconrel.2009.02.006. Epub 2009 Feb 20.
6
Fibrin hydrogels for non-viral vector delivery in vitro.纤维蛋白水凝胶用于体外非病毒载体传递。
J Control Release. 2009 Jun 5;136(2):148-54. doi: 10.1016/j.jconrel.2009.02.004. Epub 2009 Feb 20.
7
DNA delivery from matrix metalloproteinase degradable poly(ethylene glycol) hydrogels to mouse cloned mesenchymal stem cells.从基质金属蛋白酶可降解的聚乙二醇水凝胶向小鼠克隆间充质干细胞递送DNA。
Biomaterials. 2009 Jan;30(2):254-65. doi: 10.1016/j.biomaterials.2008.09.027. Epub 2008 Oct 5.
8
Fibrin: a versatile scaffold for tissue engineering applications.纤维蛋白：一种适用于组织工程应用的多功能支架。
Tissue Eng Part B Rev. 2008 Jun;14(2):199-215. doi: 10.1089/ten.teb.2007.0435.
9
Design of biodegradable hydrogel for the local and sustained delivery of angiogenic plasmid DNA.用于局部和持续递送血管生成质粒DNA的可生物降解水凝胶的设计
Pharm Res. 2008 May;25(5):1230-8. doi: 10.1007/s11095-007-9526-7. Epub 2008 Jan 9.
10
Delivery of non-viral gene carriers from sphere-templated fibrin scaffolds for sustained transgene expression.从球形模板化纤维蛋白支架递送非病毒基因载体以实现持续的转基因表达。
Biomaterials. 2007 Nov;28(31):4705-16. doi: 10.1016/j.biomaterials.2007.07.026. Epub 2007 Aug 6.
Zotero 插件