Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
Int J Syst Evol Microbiol. 2011 Dec;61(Pt 12):3028-3032. doi: 10.1099/ijs.0.025718-0. Epub 2011 Feb 4.
A novel gammaproteobacterium, designated LnR5-47(T), was isolated from soil of a ginseng field in Liaoning province, China. The isolate was a Gram-negative, aerobic, non-motile, non-spore-forming rod. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain LnR5-47(T) belonged to the genus Rhodanobacter. The isolate was most closely related to Rhodanobacter ginsengisoli GR17-7(T), Rhodanobacter terrae GP18-1(T), Dyella ginsengisoli Gsoil 3046(T), Rhodanobacter soli DCY45(T), Dyella soli JS12-10(T) and Dyella japonica IAM 15069(T) (98.0, 97.9, 97.7, 97.3, 97.2 and 97.1% 16S rRNA gene sequence similarity, respectively). Chemotaxonomic data (Q-8 as the predominant ubiquinone, and iso-C(16:0), iso-C(17:1)ω9c and iso-C(15:0) as the major fatty acids) also supported the affiliation of strain LnR5-47(T) with the genus Rhodanobacter. However, DNA-DNA relatedness between strain LnR5-47(T) and its closest phylogenetic neighbours was <25.8%. Moreover, physiological and biochemical tests phenotypically differentiated the isolate from other members of the genus Rhodanobacter. Therefore, strain LnR5-47(T) represents a novel species, for which the name Rhodanobacter panaciterrae sp. nov. is proposed; the type strain is LnR5-47(T) (=KACC 12826(T)=KCTC 22232(T)=LMG 24460(T)).
一株新的γ-变形杆菌，命名为 LnR5-47(T)，从中国辽宁省人参田的土壤中分离得到。该分离株为革兰氏阴性、需氧、非运动、非孢子形成的棒状。基于 16S rRNA 基因序列的系统发育分析表明，菌株 LnR5-47(T)属于 Rhodanobacter 属。该分离株与 Rhodanobacter ginsengisoli GR17-7(T)、Rhodanobacter terrae GP18-1(T)、Dyella ginsengisoli Gsoil 3046(T)、Rhodanobacter soli DCY45(T)、Dyella soli JS12-10(T)和 Dyella japonica IAM 15069(T)最为密切相关（98.0、97.9、97.7、97.3、97.2 和 97.1% 的 16S rRNA 基因序列相似性）。化学分类学数据（Q-8 为主要的泛醌，iso-C(16:0)、iso-C(17:1)ω9c 和 iso-C(15:0)为主要脂肪酸）也支持菌株 LnR5-47(T)与 Rhodanobacter 属的归属。然而，菌株 LnR5-47(T)与其最接近的系统发育近缘种之间的 DNA-DNA 同源性<25.8%。此外，生理生化试验在表型上区分了该分离株与 Rhodanobacter 属的其他成员。因此，菌株 LnR5-47(T)代表一个新的种，提议命名为 Panaciterrae panaciterrae sp. nov.；模式菌株为 LnR5-47(T)=KACC 12826(T)=KCTC 22232(T)=LMG 24460(T)。
