Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, 1 Seocheon-dong, Giheung-gu Yongin-si, Gyeonggi-do 449-701, Republic of Korea.
Int J Syst Evol Microbiol. 2010 Dec;60(Pt 12):2935-2939. doi: 10.1099/ijs.0.019422-0. Epub 2010 Jan 29.
Strain DCY45(T) was isolated from soil of a ginseng field in Pocheon Province, Korea. Strain DCY45(T) was Gram-negative, oxidase- and catalase-positive, motile and rod-shaped and produced yellow pigments on R2A agar. The organism grew optimally at 30 °C and at pH 7.0. The G+C content of the genomic DNA was 65.4 mol%. The predominant respiratory quinone was Q-8. The major fatty acids were iso-C(17 : 1)ω9c, iso-C(16 : 0) and iso-C(15 : 0). Phylogenetic analysis based on the 16S rRNA gene sequence was used to determine the taxonomic position of strain DCY45(T), which is most closely related to species of the genus Rhodanobacter, with similarity levels of 96.0-98.4 %; DNA-DNA relatedness with related strains was lower than 60 %. Strain DCY45(T) differed significantly from related type strains in phenotypic characteristics. On the basis of these phenotypic, genotypic and chemotaxonomic studies, strain DCY45(T) represents a novel species of the genus Rhodanobacter, for which the name Rhodanobacter soli sp. nov. is proposed. The type strain is DCY45(T) (=KCTC 22620(T) =JCM 16126(T)).
菌株 DCY45(T) 从韩国抱川人参田的土壤中分离得到。菌株 DCY45(T) 革兰氏阴性，氧化酶和过氧化氢酶阳性，可运动，呈杆状，在 R2A 琼脂上产生黄色色素。该菌在 30°C 和 pH7.0 时生长最佳。基因组 DNA 的 G+C 含量为 65.4mol%。主要呼吸醌为 Q-8。主要脂肪酸为 iso-C(17 : 1)ω9c、iso-C(16 : 0)和 iso-C(15 : 0)。根据 16S rRNA 基因序列的系统发育分析用于确定菌株 DCY45(T)的分类地位，它与 Rhodanobacter 属的种最为密切相关，相似度为 96.0-98.4%；与相关菌株的 DNA-DNA 相关性低于 60%。菌株 DCY45(T)在表型特征上与相关的模式菌株有明显的不同。基于这些表型、基因型和化学分类研究，菌株 DCY45(T)代表了 Rhodanobacter 属的一个新种，建议将其命名为 Rhodanobacter soli sp. nov.。其模式菌株为 DCY45(T) (=KCTC 22620(T) =JCM 16126(T))。
