Julius Kühn-Institut, Federal Research Centre for Cultivated Plants (JKI), Department of Epidemiology and Pathogen Diagnostics, Messeweg 11-12, 38104 Braunschweig, Germany.
Appl Environ Microbiol. 2011 Apr;77(7):2527-30. doi: 10.1128/AEM.02577-10. Epub 2011 Feb 4.
Two soils were amended three times with pig manure. The abundance of sulfonamide resistance genes was determined by quantitative PCR 2 months after each application. In both soils treated with sulfadiazine-containing manure, the numbers of copies of sul1 and sul2 significantly increased compared to numbers after treatments with antibiotic-free manure or a control and accumulated with repeated applications.
两份土壤经三次添加猪粪处理。每次添加后两个月，通过定量 PCR 确定磺胺类抗性基因的丰度。与用不含抗生素的粪肥或对照处理相比，两份添加含磺胺嘧啶粪肥的土壤中，sul1 和 sul2 的拷贝数显著增加，且随着重复添加而累积。
