高通量单分子光流分析。
High-throughput single-molecule optofluidic analysis.
机构信息
Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.
出版信息
Nat Methods. 2011 Mar;8(3):242-5. doi: 10.1038/nmeth.1569. Epub 2011 Feb 6.
Abstract
We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.
摘要
我们描述了一种高通量、自动化的单分子测量系统，配备了微流控技术。微流控混合装置集成了阀门和泵，以皮升的分辨率精确完成生物分子的滴定。我们证明，这种方法能够快速采样生物分子构象景观和酶活性，以大肠杆菌 RNA 聚合酶转录的形式，作为化学环境的函数。
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High-resolution temperature-concentration diagram of alpha-synuclein conformation obtained from a single Förster resonance energy transfer image in a microfluidic device.在微流控装置中，通过单个荧光共振能量转移图像获得的α-突触核蛋白构象的高分辨率温度-浓度图。
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