衰老加速小鼠派尔集合淋巴结的免疫组织化学检查

Immunohistochemical examination of Peyer's patches in senescence-accelerated mice.

作者信息

Yoshioka H, Takeda T, Higuchi K, Ohshio G, Miyake T, Sugiyama T, Kita T

机构信息

Department of Geriatric Medicine, Kyoto University Hospital, Japan.

出版信息

Autoimmunity. 1990;8(1):25-35. doi: 10.3109/08916939008998429.

DOI:10.3109/08916939008998429

PMID:2129782

Abstract

The distribution of T cells, B cells and murine senile amyloid protein in Peyer's Patches was examined in senescence accelerated (SAM-P/1) and control (SAM-R/1) mice ranging in age from two to ten months. An immunohistochemical detection of lymphocyte surface antigens of T cells and B cells. A murine senile amyloid protein in Peyer's patches was detected by immunohistochemical staining with the specific antiserum. Congo red staining and electron microscopy. The T cell population increased and B cell population decreased slightly with age in SAM-P/1 mice, but little change was observed with age in SAM-R/1 mice. Murine senile amyloid protein positive spots were seen surrounding the vessels of the thymus-dependent area at 6 months of age, and were observed throughout the Peyer's patches at 10 months of age, but were not observed by 10 months of age in SAM-R/1 mice.

摘要

在2至10月龄的加速衰老小鼠（SAM-P/1）和对照小鼠（SAM-R/1）中，检测了派尔集合淋巴结中T细胞、B细胞和小鼠老年淀粉样蛋白的分布。采用免疫组织化学方法检测T细胞和B细胞的淋巴细胞表面抗原。用特异性抗血清通过免疫组织化学染色、刚果红染色和电子显微镜检测派尔集合淋巴结中的小鼠老年淀粉样蛋白。在SAM-P/1小鼠中，T细胞数量随年龄增加，B细胞数量略有下降，但在SAM-R/1小鼠中，随年龄变化不大。在6月龄时，小鼠老年淀粉样蛋白阳性斑点出现在胸腺依赖区的血管周围，10月龄时在整个派尔集合淋巴结中都能观察到，但在10月龄的SAM-R/1小鼠中未观察到。