Henderson A J, Dorshkind K
Division of Biomedical Sciences, University of California, Riverside 92521-0121.
Semin Immunol. 1990 May;2(3):181-7.
B lymphocyte differentiation proceeds in the bone marrow in association with a supporting framework of stromal cells. The various long-term bone marrow cultures have provided valuable in vitro models for the study of both normal and dysregulated, stromal cell dependent B lymphopoiesis. Stromal cell lines have been cloned from the adherent layers of the cultures, and recent studies indicate these cells produce soluble factors that control the growth and differentiation of B lineage cells. Modifications of the long-term cultures are now being developed that permit specific stages in the B cell developmental pathway to be amplified. These systems should prove valuable for analysis of stromal cell derived signals that regulate the growth and differentiation of B lineage cells.
B淋巴细胞的分化在骨髓中与基质细胞的支持框架相关联地进行。各种长期骨髓培养物为研究正常和失调的、依赖基质细胞的B淋巴细胞生成提供了有价值的体外模型。已从培养物的贴壁层中克隆出基质细胞系，最近的研究表明这些细胞产生控制B系细胞生长和分化的可溶性因子。目前正在开发长期培养物的改良方法，以扩增B细胞发育途径中的特定阶段。这些系统对于分析调节B系细胞生长和分化的基质细胞衍生信号应具有重要价值。
