Ku G, Witte O N
J Immunol. 1986 Nov 1;137(9):2802-7.
A long-term in vitro culture system derived from murine bone marrow cells can successfully support the growth of B cell precursors, pre-B cells, and IgM-expressing B cells. Intermediates in the B cell developmental pathway are known to have differential sensitivities to the toxic effects of corticosteroids. We demonstrate here that long term B lineage cultures can be established with the corticosteroid-resistant cell population from bone marrow. Kinetics for the establishment and growth of cultures derived from corticosteroid-treated marrow are similar to those observed with control cultures. Cells obtained from both sets of cultures have similar morphologies and ranges of phenotypic markers. These results indicate that the cell responsible for the outgrowth of the long term B lineage cultures is corticosteroid resistant and is likely to be earlier in the B lymphocyte lineage than steroid-sensitive pre-B cells.
源自小鼠骨髓细胞的长期体外培养系统能够成功支持B细胞前体、前B细胞和表达IgM的B细胞的生长。已知B细胞发育途径中的中间体对皮质类固醇的毒性作用具有不同的敏感性。我们在此证明，可以用来自骨髓的皮质类固醇抗性细胞群体建立长期B系培养物。源自皮质类固醇处理的骨髓的培养物的建立和生长动力学与对照培养物中观察到的相似。从两组培养物中获得的细胞具有相似的形态和表型标志物范围。这些结果表明，负责长期B系培养物生长的细胞对皮质类固醇具有抗性，并且可能比类固醇敏感的前B细胞更早处于B淋巴细胞谱系中。
