天芥菜中的抗氧化性环烯醚萜苷。

Antioxidative iridoid glycosides from the sky flower (Duranta repens Linn).

机构信息

KUST Institute of Medical Sciences, Kohat University of Science and Technology, Kohat, NWFP, Pakistan.

出版信息

J Enzyme Inhib Med Chem. 2011 Feb;26(1):88-92. doi: 10.3109/14756361003724778.

Abstract

Phytochemical investigations were performed on the EtOAc-soluble fraction of the whole plant of the sky flower (Duranta repens) which led to the isolation of the iridoid glycosides 1-6. Their structures were elucidated by both 1D and 2D NMR spectroscopic analysis. All the compounds showed potent antioxidative scavenging activity in four different tests, with half maximal inhibitory concentration (IC(50)) values in the range 0.481-0.719 mM against DPPH radicals, 4.07-17.21 μM for the hydroxyl radical (·OH) inhibitory activity test, 43.3-97.37 μM in the total reactive oxygen species (ROS) inhibitory activity test, and 3.39-18.94 μM in the peroxynitrite (ONOO(-)) scavenging activity test. Duranterectoside A (1) displayed the strongest scavenging potential with IC(50) values of (0.481 ± 0.06 mM, 4.07 ± 0.03, 43.30 ± 0.05, 3.39 ± 0.02 μM) for the DPPH radicals, ·OH inhibitory activity test, total ROS inhibitory activity test and the ONOO(-) scavenging activity test, respectively.

摘要

植物化学研究对蓝星花（垂花悬铃花）全株的 EtOAc 可溶部分进行了研究，从中分离得到了 6 个环烯醚萜苷类化合物 1-6。通过 1D 和 2D NMR 光谱分析确定了它们的结构。所有化合物在四项不同的测试中均表现出较强的抗氧化清除活性，其对 DPPH 自由基的半最大抑制浓度（IC50）值在 0.481-0.719 mM 之间，对羟基自由基（·OH）抑制活性测试的 IC50 值为 4.07-17.21 μM，对总活性氧（ROS）抑制活性测试的 IC50 值为 43.3-97.37 μM，对过氧亚硝酸盐（ONOO（-））清除活性测试的 IC50 值为 3.39-18.94 μM。垂序商陆苷 A（1）表现出最强的清除能力，其对 DPPH 自由基、·OH 抑制活性测试、总 ROS 抑制活性测试和 ONOO（-）清除活性测试的 IC50 值分别为（0.481 ± 0.06 mM、4.07 ± 0.03、43.30 ± 0.05、3.39 ± 0.02 μM）。