Padmini Ekambaram, Lavanya Srinivasan
P.G. Department of Biochemistry, Bharathi Women's College, Chennai, Tamilnadu, India.
Aust N Z J Obstet Gynaecol. 2011 Feb;51(1):47-52. doi: 10.1111/j.1479-828X.2010.01246.x. Epub 2010 Dec 7.
BACKGROUND AIM: Pre-eclampsia is a hypertensive disorder characterised by endothelial dysfunction and is one of the leading cause of preterm birth. Increasing oxidative stress and antioxidant disequilibria promote apoptosis and may play a defining role in the development of pre-eclampsia. Heat shock protein 70 (HSP70) is an effective molecular chaperone, playing a role in cell protection from damage in response to stress stimuli. This study focus on the expression of HSP70 in endothelial cells during pre-eclampsia compared with normotensive pregnant women.
The conjugated diene (CD), lipid hydro peroxide (LHP), total antioxidant capacity (TAC) analysis and glutathione redox ratio (GRR) are the stress specific parameters assessed in the placental endothelial cells of normotensive and pre-eclamptic subjects to evaluate the oxidative stress status. As the synthesis of HSP70 is regulated by a transcription factor, heat shock factor 1 (HSF1), its expression was also analysed.
There was a significant decrease in the viability (P < 0.05) of the endothelial cells isolated from pre-eclamptic subjects compared to normotensive subjects. The level of CD (P < 0.01) and LHP (P < 0.01) was increased with a simultaneous decrease in GRR (P < 0.05) and TAC (P < 0.05) levels in the endothelial cells of pre-eclamptic subjects compared to normotensive subjects. The expression of HSP70 (P < 0.05) and HSF1 (P < 0.05) was increased in pre-eclamptic subjects compared to normotensive subjects.
The over expression of HSP70 and HSF1 seen in the pre-eclamptic endothelial cells suggests its possible protective role as stress specific natural adaptive response against the generated stress.
背景目的：子痫前期是一种以血管内皮功能障碍为特征的高血压疾病，是早产的主要原因之一。氧化应激增加和抗氧化失衡会促进细胞凋亡，并可能在子痫前期的发展中起决定性作用。热休克蛋白70（HSP70）是一种有效的分子伴侣，在细胞受到应激刺激时保护细胞免受损伤。本研究聚焦于子痫前期患者与血压正常孕妇相比，内皮细胞中HSP70的表达情况。
共轭二烯（CD）、脂质过氧化氢（LHP）、总抗氧化能力（TAC）分析以及谷胱甘肽氧化还原比（GRR）是在血压正常和子痫前期患者的胎盘内皮细胞中评估的应激特异性参数，以评估氧化应激状态。由于HSP70的合成受转录因子热休克因子1（HSF1）调控，因此也对其表达进行了分析。
与血压正常的受试者相比，子痫前期受试者分离出的内皮细胞活力显著降低（P < 0.05）。与血压正常的受试者相比，子痫前期受试者内皮细胞中CD水平（P < 0.01）和LHP水平（P < 0.01）升高，同时GRR水平（P < 0.05）和TAC水平（P < 0.05）降低。与血压正常受试者相比，子痫前期受试者中HSP70（P < 0.05）和HSF1（P < 0.05）的表达增加。
子痫前期内皮细胞中HSP70和HSF1的过表达表明其可能作为针对所产生应激的应激特异性自然适应性反应发挥保护作用。
