National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Bioresour Technol. 2011 Apr;102(7):4787-92. doi: 10.1016/j.biortech.2011.01.015. Epub 2011 Jan 14.
Plant cell wall is the most abundant substrate for bioethanol production, and plants also represent a key resource for glycoside hydrolase (GH). To exploit efficient way for bioethanol production with lower cellulase loading, the potential of plant GH for lignocellulose bioconversion was evaluated. The GH activity for cell wall proteins (CWPs) was detected from fresh corn stover (FCS), and the synergism of which with Trichoderma reesei cellulase was also observed. The properties for the GH of FCS make it a promising enzyme additive for lignocellulose biodegradation. To make use of the plant GH, novel technology for hydrolysis and ethanol fermentation was developed with corn stover as substrate. Taking steam-exploded corn stover as substrate for hydrolysis and ethanol fermentation, compared with T. reesei cellulase loaded alone, the final glucose and ethanol accumulation increased by 60% and 63% respectively with GH of FCS as an addition.
植物细胞壁是生物乙醇生产最丰富的底物，而植物也是糖苷水解酶（GH）的重要资源。为了利用纤维素酶用量较低的高效方法生产生物乙醇，评估了植物 GH 对木质纤维素生物转化的潜力。从新鲜玉米秸秆（FCS）中检测到细胞壁蛋白（CWPs）的 GH 活性，并观察到其与里氏木霉纤维素酶的协同作用。FCS 的 GH 特性使其成为木质纤维素生物降解有前途的酶添加剂。为了利用植物 GH，以玉米秸秆为底物开发了水解和乙醇发酵的新技术。以蒸汽爆破玉米秸秆为水解和乙醇发酵的底物，与单独加载里氏木霉纤维素酶相比，添加 FCS 的 GH 可使最终葡萄糖和乙醇积累分别增加 60%和 63%。
