Enza Zaden Research and Development B.V, P.O. Box 7, 1600 AA Enkhuizen, The Netherlands.
Plant Cell Rep. 2011 Jun;30(6):1107-15. doi: 10.1007/s00299-011-1018-x. Epub 2011 Feb 9.
Pepper (Capsicum L.) is a nutritionally and economically important crop that is cultivated throughout the world as a vegetable, condiment, and food additive. Genetic transformation using Agrobacterium tumefaciens (agrobacterium) is a powerful biotechnology tool that could be used in pepper to develop community-based functional genomics resources and to introduce important agronomic traits. However, pepper is considered to be highly recalcitrant for agrobacterium-mediated transformation, and current transformation protocols are either inefficient, cumbersome or highly genotype dependent. The main bottleneck in pepper transformation is the inability to generate cells that are competent for both regeneration and transformation. Here, we report that ectopic expression of the Brassica napus BABY BOOM AP2/ERF transcription factor overcomes this bottleneck and can be used to efficiently regenerate transgenic plants from otherwise recalcitrant sweet pepper (C. annuum) varieties. Transient activation of BABY BOOM in the progeny plants induced prolific cell regeneration and was used to produce a large number of somatic embryos that could be converted readily to seedlings. The data highlight the utility of combining biotechnology and classical plant tissue culture approaches to develop an efficient transformation and regeneration system for a highly recalcitrant vegetable crop.
辣椒（Capsicum L.）是一种营养丰富、经济价值高的作物，作为蔬菜、调味品和食品添加剂在世界各地广泛种植。利用根癌农杆菌（agrobacterium）进行遗传转化是一种强大的生物技术工具，可以用于开发基于社区的功能基因组资源，并引入重要的农艺性状。然而，辣椒被认为对农杆菌介导的转化高度顽固，目前的转化方案要么效率低下，要么繁琐，要么高度依赖基因型。辣椒转化的主要瓶颈是无法生成同时具有再生和转化能力的细胞。在这里，我们报告说，异位表达芸薹属 Brassica napus BABY BOOM AP2/ERF 转录因子克服了这一瓶颈，并可用于有效地从其他顽固的甜椒（C. annuum）品种再生转基因植物。BABY BOOM 在后代植物中的瞬时激活诱导了大量细胞再生，并用于产生大量可以很容易转化为幼苗的体细胞胚。这些数据突出了将生物技术和经典植物组织培养方法相结合，开发高效转化和再生系统的实用性，以用于高度顽固的蔬菜作物。
