Rui Y C, Zhang D Z, Sun D X, Zeng G Q
Department of Pharmacology, Faculty of Pharmacy, Second Military Medical University, Shanghai, China.
Zhongguo Yao Li Xue Bao. 1990 Sep;11(5):418-21.
Brain ischemia was produced by occluding bilateral common carotid arteries of Mongolia gerbil with clips for 30-min and reperfusion was established by removing the clips. Superoxide dismutase (SOD) in brain tissue was measured by chemoluminescence, malondialdehyde (MDA) by fluorescence spectrometry and leukotrienes (LT) by bioassay. Decrease of SOD and increase of MDA were significant in the brain after 30-min ischemia followed by 2-min reperfusion; The level of SOD increased from 13.4 +/- 2.7 to 18.8 +/- 3.0, 19.8 +/- 2.5, 22.1 +/- 3.9 x 10(3) units/g brain tissue and MDA decreased from 218 +/- 26 to 169 +/- 41, 167 +/- 36, 167 +/- 44 nmol/g brain tissue respectively by ip silybin 100, 200 and 400 mg/kg 30-min before occlusion. LT decreased from 3.1 +/- 1.0 to 1.5 +/- 0.4 ng/g brain tissue after ip silybin 200 mg/kg. It suggested that the protective effect of silybin on ischemic brain result from the inhibition of 5-lipoxygenase and lipoperoxide and scavenging of oxygen free radical.
通过用夹子夹闭蒙古沙鼠双侧颈总动脉30分钟造成脑缺血，然后移除夹子建立再灌注。采用化学发光法测定脑组织中的超氧化物歧化酶（SOD），荧光光谱法测定丙二醛（MDA），生物测定法测定白三烯（LT）。缺血30分钟后再灌注2分钟，脑组织中SOD降低，MDA升高；在夹闭前30分钟腹腔注射水飞蓟宾100、200和400mg/kg，脑组织中SOD水平分别从13.4±2.7升高至18.8±3.0、19.8±2.5、22.1±3.9×10（3）单位/克脑组织，MDA分别从218±26降至169±41、167±36、167±44nmol/克脑组织。腹腔注射水飞蓟宾200mg/kg后，LT从3.1±1.0降至1.5±0.4ng/克脑组织。提示水飞蓟宾对缺血脑的保护作用源于对5-脂氧合酶和脂质过氧化物的抑制以及对氧自由基的清除。
