Department of Dental Materials and Biomaterials Research, RWTH Aachen University Hospital, Pauwelsstraße 30, 52074 Aachen, Germany.
J Mater Sci Mater Med. 2011 Apr;22(4):763-71. doi: 10.1007/s10856-011-4252-4. Epub 2011 Feb 10.
A functionalization is required for calcium phosphate-based bone substitute materials to achieve an entire bone remodeling. In this study it was hypothesized that a tailored composite of tricalcium phosphate and a bioactive glass can be loaded sufficiently with rhBMP-2 for functionalization. A composite of 40 wt% tricalcium phosphate and 60 wt% bioactive glass resulted in two crystalline phases, wollastonite and rhenanite after sintering. SEM analysis of the composite's surface revealed a spongious bone-like morphology after treatment with different acids. RhBMP-2 was immobilized non-covalently by treating with chrome sulfuric acid (CSA) and 3-aminopropyltriethoxysilane (APS) and covalently by treating with CSA/APS, and additionally with 1,1'-carbonyldiimidazole. It was proved that samples containing non-covalently immobilized rhBMP-2 on the surface exhibit significant biological activity in contrast to the samples with covalently bound protein on the surface. We conclude that a tailored composite of tricalcium phosphate and bioactive glass can be loaded sufficiently with BMP-2.
为了使磷酸钙基骨替代材料实现完全的骨重塑，需要进行功能化。在这项研究中，我们假设可以将磷酸三钙和生物活性玻璃的定制复合材料充分负载 rhBMP-2 以进行功能化。经过烧结，40wt%磷酸三钙和 60wt%生物活性玻璃的复合材料形成了两种晶体相，即硅灰石和硅铍石。经过不同酸处理后，复合材料表面的 SEM 分析显示出类似海绵骨的形态。通过用铬硫酸（CSA）和 3-氨基丙基三乙氧基硅烷（APS）进行非共价处理，以及用 CSA/APS 并用 1,1'-碳化二亚胺进行共价处理，可以将 rhBMP-2 固定在表面上。事实证明，与表面结合有共价结合蛋白质的样品相比，表面结合有非共价固定 rhBMP-2 的样品表现出显著的生物活性。我们得出结论，磷酸三钙和生物活性玻璃的定制复合材料可以充分负载 BMP-2。
