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水溶液中紫霉素的1H、13C和15N核磁共振谱中氢-氘交换的定性方面。

Qualitative aspects of hydrogen-deuterium exchange in the 1H, 13C, and 15N nuclear magnetic resonance spectra of viomycin in aqueous solution.

作者信息

Hawkes G E, Randall E W, Hull W E, Gattegno D, Conti F

出版信息

Biochemistry. 1978 Sep 19;17(19):3986-92. doi: 10.1021/bi00612a017.

DOI:10.1021/bi00612a017
PMID:213101
Abstract

The 1H, 13C, and 15N high field nuclear magnetic resonance spectra of the cyclic peptide viomycin have been fully assigned using homo- and heteronuclear double resonance experiments and pH effects. In addition it is shown how the two- and three-bond H-D isotope effects upon carbonyl resonances may assist in their assignment. The resistance to exchange with solvent water of the amide proton involved in the transannular hydrogen bond is observed directly in the 1H spectra, via the isotope effect on a carbonyl resonance in the 13C spectra, and via the one-bond 1H couppling in the 15N spectra.

摘要

利用同核和异核双共振实验以及pH效应，已对环肽紫霉素的1H、13C和15N高场核磁共振谱进行了全归属。此外，还展示了羰基共振上的二键和三键H-D同位素效应如何有助于其归属。通过1H谱中对跨环氢键中酰胺质子与溶剂水交换的抗性、13C谱中羰基共振上的同位素效应以及15N谱中的一键1H耦合，可直接观察到该抗性。

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