Woody C D, Swartz B E, Gruen E
Brain Res. 1978 Dec 15;158(2):373-95. doi: 10.1016/0006-8993(78)90682-0.
The effects on input resistance (Rm) of extracellular iontophoresis of acetylcholine (ACh) and of intracellular iontophoresis of cyclic GMP (cGMP) were studied in neurons of the coronal-pericruciate cortex of awake cats. Control studies were also conducted including iontophoresis of saline extracellularly and 5'-GMP intracellularly. (1) Substantial increases in Rm occurred in approximately half the neurons given ACh or cGMP. (2) In the absence of associated repetitive spike discharge induced by intracellular injection of depolarizing current pulses during iontophoretic applications, the increases in Rm were transient occurring in less than 30 sec and lasting 4--5 min. (3) With associated current-induced spike discharge, the increases in Rm persisted for as long as the neurons could be held -- up to 1.5 h maximally. (4) Rm was not increased if saline was substituted for ACh, if 5'-GMP was substituted for cGMP, or if the neuron was only discharged repeatedly. (5) The magnitude and time course of both transient and persistent increases in Rm were comparable between cells given ACh or cGMP and whether action potentials and resting potentials were greater than or equal to 40 mV (average 47 mV)* or were less.
在清醒猫的冠状-十字周皮层神经元中，研究了细胞外离子导入乙酰胆碱（ACh）和细胞内离子导入环磷酸鸟苷（cGMP）对输入电阻（Rm）的影响。还进行了对照研究，包括细胞外离子导入生理盐水和细胞内离子导入5'-GMP。（1）给予ACh或cGMP的神经元中，约一半的神经元Rm显著增加。（2）在离子导入应用期间，若在细胞内注入去极化电流脉冲未诱发相关的重复锋电位发放，Rm的增加是短暂的，不到30秒出现，持续4 - 5分钟。（3）若伴有电流诱发的锋电位发放，Rm的增加会持续到神经元能够维持的最长时间——最长可达1.5小时。（4）若用生理盐水替代ACh、用5'-GMP替代cGMP，或仅使神经元重复放电，Rm不会增加。（5）给予ACh或cGMP的细胞，以及动作电位和静息电位大于或等于40 mV（平均47 mV）*或更低时，Rm的短暂和持续增加的幅度及时程相当。
