Swartz B E, Woody C D
Exp Neurol. 1984 Nov;86(2):388-404. doi: 10.1016/0014-4886(84)90195-x.
Intracellular injection of specific antibody to cyclic 3',5'-guanosine monophosphate (cGMP-Ab) produced substantial decreases in input resistance (Rm) selectively in neurons of the motor cortex that had responded with increased resistance to prior application of muscarinic agents. Intracellular injection of nonspecific immunoglobulins (IgG) did not produce this effect. (Some nonspecific effects on spike production occurred in cells given IgG or cGMP-Ab.) The decrease in Rm may be interpreted as being consequential to a reduction in baseline amounts of active cGMP due to binding of cGMP with the injected antibody. In cells which demonstrated a prior increase in Rm following extracellular application of the muscarinic agonist, aceclidine, or acetylcholine, injection of antibody to cGMP also resulted in suppression of the increase in Rm to subsequent applications of these muscarinic agents. Some increases in firing rate to these agents continued to be observed after injection of cGMP-Ab. The results support the hypothesis that cGMP mediates effects of muscarinic neurotransmission on the conductances of neurons of the motor cortex of awake cats. Intracellular injection of antibodies to specific cellular elements is shown to be feasible in cortical neurons of awake cats and may prove a useful adjunct to future studies of neurotransmitter mechanisms.
向细胞内注射特异性抗环磷酸鸟苷（cGMP）抗体（cGMP-Ab），选择性地使运动皮层中那些对先前应用毒蕈碱剂反应为电阻增加的神经元的输入电阻（Rm）大幅降低。向细胞内注射非特异性免疫球蛋白（IgG）则不会产生这种效应。（给予IgG或cGMP-Ab的细胞中出现了一些对动作电位产生的非特异性效应。）Rm的降低可能被解释为由于cGMP与注入的抗体结合，导致活性cGMP的基线量减少所致。在细胞外应用毒蕈碱激动剂醋甲胆碱或乙酰胆碱后，其Rm先前出现增加的细胞中，注射cGMP抗体也会抑制这些毒蕈碱剂后续应用时Rm的增加。注射cGMP-Ab后，仍可观察到对这些药物的一些放电频率增加。这些结果支持这样的假说，即cGMP介导了毒蕈碱神经传递对清醒猫运动皮层神经元电导的影响。向清醒猫的皮层神经元内注射针对特定细胞成分的抗体已证明是可行的，并且可能被证明是未来神经递质机制研究的有用辅助手段。
