罗斯特氏集落试验：细胞免疫的体外相关物。

T rosette assay: in vitro correlate of cellular immunity.

机构信息

James Buchanan Brady Urological Institute, The Johns Hopkins Hospital, Baltimore, Maryland 21205, USA.

出版信息

Urology. 1974 Jul;4(1):100-4. doi: 10.1016/0090-4295(74)90119-8.

Abstract

The T rosette assay, based on the capacity of thymus-derived lymphocytes (mediators of tumor-inhibiting immunity) to bind sheep erythrocytes in vitro, was used to quantitate percentages of T rosette-forming lymphocytes in the peripheral blood of 38 patients with urologic cancer. Results obtained using the T rosette assay yielded statistically significant direct correlations with those obtained using two other assays which have been widely employed to monitor cellular immunity: elicitation of delayed hypersensitivity to DNCB (dinitrochlorobenzene) and the proliferative response of lymphocytes to PHA (phytohemagglutinin). The findings suggest the T rosette assay provides a meaningful in vitro correlate of cellular immunity.

摘要

T 花环试验，基于胸腺衍生淋巴细胞（肿瘤抑制免疫的介质）在体外结合绵羊红细胞的能力，用于定量测定 38 例泌尿系统癌症患者外周血中 T 花环形成淋巴细胞的百分比。使用 T 花环试验获得的结果与使用两种广泛用于监测细胞免疫的其他试验获得的结果具有统计学上的显著直接相关性：DNCB（二硝基氯苯）引起的迟发性超敏反应和淋巴细胞对 PHA（植物血凝素）的增殖反应。这些发现表明 T 花环试验提供了细胞免疫的有意义的体外相关性。