Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala-147002, Punjab, India.
Physiol Behav. 2011 Jun 1;103(3-4):321-9. doi: 10.1016/j.physbeh.2011.02.014. Epub 2011 Feb 12.
The present study was designed to investigate whether adaptogenic factors may be transferred from stress adapted rats to naïve rats and to explore the nature of endogenous adaptogens by pharmacological modulation. The rats were subjected to cold water immersion stress by placing them individually in a tank of water (depth=15.5cm; temperature=16±2°C) for 5min. The rats were subjected to single episode of cold water immersion stress for acute stress; while for adaptation, the rats were subjected to repeated episodes of same stressor for 5 consecutive days. The plasma of stress adapted rats was administered to naïve rats before subjecting to acute stress. The stress related behavioral alterations were assessed using the actophotometer, the hole board, the open field and the social interaction tests. Acute stress with single episode of cold water immersion was associated with behavioral alterations. However, the behavioral alterations were significantly restored on subjecting repeated episodes of cold water immersion. Administration of plasma of stress adapted rats also attenuated acute stress associated behavioral alterations. Administration of naltrexone abolished the restoration of behavioral changes as a part of adaptive process in repeated stress subjected rats as well as the anti-stress effects of plasma of stress adapted rat. It may be concluded that opioids may be the potential endogenous adaptogens that tend to restore the homeostasis during repeated episodes of stress. Furthermore, the endogenous adaptogens may be transferred in the form of plasma from repeated stress subjected rats to the naïve rats to confer the anti-stress properties.
本研究旨在探讨应激适应大鼠的适应原是否可以转移到未适应大鼠体内，并通过药理学调节来探索内源性适应原的性质。将大鼠单独放入水深 15.5cm、水温 16±2°C 的水箱中 5 分钟，使其遭受冷水浸泡应激。大鼠单次遭受冷水浸泡应激为急性应激；而对于适应，大鼠连续 5 天接受相同应激源的重复发作。在急性应激前，将应激适应大鼠的血浆给予未适应大鼠。使用活动记录仪、洞板、旷场和社交互动测试评估与应激相关的行为改变。单次冷水浸泡急性应激与行为改变有关。然而，重复冷水浸泡应激可显著恢复这些行为改变。应激适应大鼠的血浆给药也可减轻急性应激相关的行为改变。纳曲酮给药消除了重复应激大鼠适应性过程中行为变化的恢复，以及应激适应大鼠血浆的抗应激作用。可以得出结论，阿片类物质可能是潜在的内源性适应原，有助于在重复应激发作期间恢复体内平衡。此外，内源性适应原可能以血浆的形式从反复应激大鼠转移到未应激大鼠，从而赋予其抗应激特性。
