Master's in Natural Resource Management, From the Biological Research Center of the Autonomous University of the State of Morelos, Cuernavaca, Mexico.
Mycology Laboratory, Centro de Investigaciones Biológicas, Universidad Autónoma del Estado de Morelos, C.P. 62209, Cuernavaca, Morelos, Mexico.
Acta Parasitol. 2021 Sep;66(3):969-976. doi: 10.1007/s11686-021-00344-0. Epub 2021 Mar 17.
In the present study, the in vitro and in vivo effect of the hydroalcoholic extract of Neolentinus ponderosus (EHNP) on L larvae of Haemonchus contortus was evaluated.
The N. ponderosus fungus was cultivated in potato dextrose liquid medium for 7 days at 120 rpm and 25 °C. Subsequently, the EHNP was obtained; in vitro bioassays were performed in 96-well plates. Furthermore, an in vitro confrontation with different concentrations of EHNP was carried out at 72 h against L3 larvae of H. contortus. The controls used were distilled water and ivermectin at 5 mg/mL. Subsequently, the in vivo activity of EHNP was evaluated using the gerbil against H. contortus L3 larvae as an experimental model. The experimental design consisted of four groups with: (1) distilled water, (2) fenbendazole at 20 mg/kg of body weight, (3) EHNP at a dose of 81 µg/mL, and (4) EHNP at a dose of 40 µg/mL.
In vitro study showed 97% mortality of the parasite H. contortus at a concentration of 3.4 mg/mL and a lethal concentration (LC90) of 2 mg/mL EHNP. In the in vivo assessment the highest mortality was (49%) at 72 h at a concentration of 81 µg/mL bw.
The result of the present study shows that EHNP has nematicidal activity in vitro and in vivo tests (close to 97% and 50%, respectively), the fungus N. ponderosus should be considered in future tests to elucidate the secondary metabolites through spectroscopic studies.
本研究评估了皱盖假小牛肝菌（Neolentinus ponderosus）水醇提取物（EHNP）对捻转血矛线虫（Haemonchus contortus）幼虫 L 的体外和体内作用。
在 120rpm 和 25°C 的马铃薯葡萄糖液体培养基中培养 N. ponderosus 真菌 7 天。随后，获得 EHNP；在 96 孔板中进行体外生物测定。此外，在 72 小时内，用不同浓度的 EHNP 与 H. contortus L3 幼虫进行体外对抗。对照物为蒸馏水和 5mg/mL 的伊维菌素。随后，使用沙鼠作为实验模型评估 EHNP 的体内活性。实验设计包括四组：（1）蒸馏水，（2）芬苯达唑 20mg/kg 体重，（3）EHNP 剂量为 81µg/mL，（4）EHNP 剂量为 40µg/mL。
体外研究显示，寄生虫捻转血矛线虫在 3.4mg/mL 浓度下死亡率为 97%，LC90 浓度为 2mg/mL 的 EHNP。在体内评估中，浓度为 81µg/mL bw 时，72 小时时死亡率最高（49%）。
本研究结果表明，EHNP 在体外和体内试验中具有杀线虫活性（分别接近 97%和 50%），皱盖假小牛肝菌应在未来的试验中考虑，以通过光谱研究阐明其次级代谢物。
