Department of Ophthalmology, University Hospital Zurich (USZ), Zurich, Switzerland.
Invest Ophthalmol Vis Sci. 2011 May 18;52(6):3338-44. doi: 10.1167/iovs.10-6611.
To evaluate the impact of Spectralis self-acting eye-tracking (eye tracker) and retest software on the reproducibility of retinal nerve fiber layer (RNFL) thickness measurements in glaucomatous and healthy control eyes by SD-OCT.
RNFL thickness was measured in 56 normal and 47 glaucomatous eyes by one operator within one session with a brief rest between measurements. Three measurements were taken with the eye tracker and the retest function engaged (method A), and three measurements were taken without the eye tracker and without the retest function (method B). Method A and B measurements were taken alternately.
Reliability, measured by intraclass correlation coefficient (ICC) for absolute agreement and coefficient of variation (COV), was calculated for the global mean RNFL thickness (G), for each sector and for the peripapillary bundle. The ICC (and lower 95% confidence interval [CI]) for the global mean RNFL thickness (G) for method A measurements in both normal and glaucomatous eyes was 0.99 (0.98 CI). In glaucomatous eyes, the COV for method B measurements was between 2.7% and 10.5%, and between 1.3% and 3.5% for method A measurements.
The reproducibility of RNFL measurements with Spectralis SD-OCT is excellent in both normal and glaucomatous eyes and can be significantly improved by using the eye tracker and retest software. The gain of reproducibility by using the software is significantly higher in glaucomatous eyes than in normal eyes. These findings suggest that software applications are capable of significantly improving the reproducibility of RNFL thickness measurements.
通过频域光学相干断层扫描（SD-OCT）评估 Spectralis 自动眼球跟踪（眼动仪）和重新测试软件对青光眼和正常对照眼视网膜神经纤维层（RNFL）厚度测量的可重复性的影响。
由同一位操作者在一次检查中测量 56 只正常眼和 47 只青光眼眼中的 RNFL 厚度，两次测量之间有短暂休息。在启用眼动仪和重新测试功能的情况下（方法 A）进行 3 次测量，在不启用眼动仪和重新测试功能的情况下（方法 B）进行 3 次测量，两种方法交替进行。
用组内相关系数（ICC）测量绝对一致性和变异系数（COV）来评估整体平均 RNFL 厚度（G）、每个象限和视盘周围束的可靠性。在正常眼和青光眼眼中，方法 A 测量的整体平均 RNFL 厚度（G）的 ICC（和 95%置信区间[CI]下限）分别为 0.99（0.98 CI）。在青光眼眼中，方法 B 测量的 COV 为 2.7%10.5%，方法 A 测量的 COV 为 1.3%3.5%。
Spectralis SD-OCT 测量的 RNFL 重复性在正常眼和青光眼眼中均非常好，通过使用眼动仪和重新测试软件可以显著提高。在青光眼眼中，使用软件提高可重复性的效果明显高于正常眼。这些发现表明，软件应用能够显著提高 RNFL 厚度测量的可重复性。
