从异体脐带血干细胞移植而来的人胰岛中胰岛素表达细胞的低频率。
A low frequency of pancreatic islet insulin-expressing cells derived from cord blood stem cell allografts in humans.
机构信息
Larry L. Hillblom Islet Research Center, David Geffen School of Medicine, University of California Los Angeles, 900A Weyburn Place, Los Angeles, CA 90095, USA.
出版信息
Diabetologia. 2011 May;54(5):1066-74. doi: 10.1007/s00125-011-2071-2. Epub 2011 Feb 18.
AIMS/HYPOTHESIS: We sought to establish if stem cells contained in cord blood cell allografts have the capacity to differentiate into insulin-expressing beta cells in humans.
METHODS
We studied pancreases obtained at autopsy from individuals (n = 11) who had prior opposite-sex cord blood transplants to reconstitute haematopoiesis. Pancreatic tissue sections were stained first by XY-fluorescence in situ hybridisation and then insulin immunohistochemistry. Pancreases obtained at autopsy from participants without cord blood cell infusions served as controls (n = 11).
RESULTS
In the men with prior transplant of female cord blood, there were 3.4 ± 0.3% XX-positive insulin-expressing islet cells compared with 0.32 ± 0.05% (p < 0.01) in male controls. In women with prior transplant of male cord blood cells we detected 1.03 ± 0.20% XY insulin-expressing islet cells compared with 0.03 ± 0.03 in female controls (p < 0. 001).
CONCLUSIONS/INTERPRETATION: Cord blood stem cells have the capacity to differentiate into insulin-expressing cells in non-diabetic humans. It remains to be established whether these cells have the properties of beta cells.
目的/假设：我们试图确定脐带血细胞同种异体移植物中的干细胞是否有能力在人类中分化为表达胰岛素的β细胞。
方法
我们研究了 11 名个体的尸检胰腺组织，这些个体先前接受了异性脐带血移植以重建造血。胰腺组织切片首先通过 XY-荧光原位杂交然后进行胰岛素免疫组织化学染色。没有接受脐带血细胞输注的参与者的尸检胰腺作为对照（n=11）。
结果
在先前接受女性脐带血移植的男性中，有 3.4±0.3%的 XX 阳性胰岛素表达胰岛细胞，而男性对照组为 0.32±0.05%（p<0.01）。在先前接受男性脐带血细胞移植的女性中，我们检测到 1.03±0.20%的 XY 胰岛素表达胰岛细胞，而女性对照组为 0.03±0.03%（p<0.001）。
结论/解释：脐带血干细胞有能力在非糖尿病患者中分化为表达胰岛素的细胞。这些细胞是否具有β细胞的特性仍有待确定。
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