Labgenomics Clinical Laboratories; the Department of Laboratory Medicine, Inje University College of Medicine, Seoul Paik Hospital, Seoul, Korea.
Transfusion. 2011 Aug;51(8):1840-6. doi: 10.1111/j.1537-2995.2010.03056.x. Epub 2011 Feb 18.
This study was performed to determine the prevalence of antibodies to hepatitis B core antigen (anti-HBc) among Korean blood donors and frequencies of hepatitis B virus (HBV) DNA and antibodies to hepatitis B surface antigen (anti-HBs) in anti-HBc-positive donors.
A total of 12,461 consenting blood donors were consecutively enrolled from Korean Red Cross Blood Services from April to October 2008. All of the donors were screened for anti-HBc with an electrochemiluminescence immunoassay. Repeat-reactive anti-HBc-positive donors were assayed for anti-HBs and for HBV DNA using a multiplex test (Cobas TaqScreen, Roche Molecular Systems) on individual donation.
Of the 12,461 donors, 1682 (13.5%) were reactive for anti-HBc. Among different age groups, there was a steady increase in the anti-HBc-positive rate, ranging from 2.0% in the age group of less than 20 years to 80.0% in the age group of 60 years and older (p<0.0001). Of the anti-HBc-positive donors, 1523 (90.5%) were anti-HBs positive. HBV DNA was detected in two donors who were anti-HBc positive and hepatitis B surface antigen negative. The prevalence of occult HBV infection was 0.016%, and the HBV nucleic acid test (NAT) yield was 1 in 838 (0.12%).
This study helps to determine the current status of hepatitis B infection and the prevalence of occult HBV infection in the blood donor population in Korea. We estimate that in Korea, up to 161 units per million donated units from blood donors may contain HBV DNA. Although the potential infectivity of these units has been debated upon, the HBV NAT assay could prevent certain transfusion-transmitted HBV infections.
本研究旨在确定韩国献血者中乙型肝炎核心抗原抗体（抗-HBc）的流行率，以及抗-HBc 阳性献血者中乙型肝炎病毒（HBV）DNA 和乙型肝炎表面抗原抗体（抗-HBs）的频率。
2008 年 4 月至 10 月，连续从韩国红十字会血液服务处招募了 12461 名同意的献血者。所有献血者均采用电化学发光免疫分析法筛查抗-HBc。对重复反应性抗-HBc 阳性献血者进行抗-HBs 和 HBV DNA 检测，采用单个样本的多重检测（罗氏分子系统 Cobas TaqScreen）。
在 12461 名献血者中，有 1682 名（13.5%）对抗-HBc 呈反应性。在不同年龄组中，抗-HBc 阳性率呈稳步上升趋势，从 20 岁以下年龄组的 2.0%到 60 岁及以上年龄组的 80.0%（p<0.0001）。在抗-HBc 阳性献血者中，有 1523 名（90.5%）抗-HBs 阳性。有两名抗-HBc 阳性且乙型肝炎表面抗原阴性的献血者检测到 HBV DNA。隐匿性乙型肝炎感染的患病率为 0.016%，乙型肝炎核酸检测（NAT）的检出率为每 838 份样本中 1 份（0.12%）。
本研究有助于确定韩国献血人群中乙型肝炎感染的现状和隐匿性乙型肝炎感染的流行率。我们估计，在韩国，每百万献血单位中可能有多达 161 个单位含有 HBV DNA。虽然这些单位的潜在传染性存在争议，但 HBV NAT 检测可预防某些输血传播的乙型肝炎感染。
