Laboratory of Pulmonary Pharmacology, UPRES EA220, Foch Hospital, University Versailles - Saint Quentin en Yvelines, Suresnes, France.
Peptides. 2011 May;32(5):1027-34. doi: 10.1016/j.peptides.2011.02.012. Epub 2011 Feb 18.
Tachykinins are a family of structurally related peptides, including substance P (SP), hemokinin-1 (HK-1), neurokinin A (NKA), and neurokinin B. SP and NKA have been shown to modulate hematopoiesis and rat/mouse HK-1 has been found to be involved in the survival and differentiation of mouse B-cells. This study was designed to assess the expression of tachykinins with a focus on human HK-1 (hHK-1) in human B lymphocytes and the role of these peptides in cell differentiation, apoptosis and proliferation. Expression of tachykinin and tachykinin receptor mRNA was determined quantitatively in human B lymphoproliferative malignancies and compared to normal B-cells. Expression of hHK-1 and NK(1) receptor, but not SP, was detected in human B-lymphocytes, and was up-regulated in B-lymphocytes from chronic lymphocytic leukemia and non-Hodgkin's lymphoma, while it was down-regulated in acute lymphoblastic leukemia. Moreover, hHK-1, in contrast to SP, was able to induce proliferation of human pre-B lymphocytes through a NK(1) receptor-independent mechanism. These data suggest a role for hHK-1 in normal and pathological B lymphopoiesis, and open the door to a better understanding of the physiopathological mechanisms leading to lymphoproliferative malignancies.
速激肽是一类结构相关的肽，包括 P 物质（SP）、血激肽-1（HK-1）、神经激肽 A（NKA）和神经激肽 B。已经证明 SP 和 NKA 可以调节造血，并且已经发现鼠 HK-1 参与了小鼠 B 细胞的存活和分化。本研究旨在评估速激肽的表达，重点是人类 B 淋巴细胞中的人 HK-1（hHK-1），以及这些肽在细胞分化、凋亡和增殖中的作用。定量检测了人类 B 淋巴细胞增殖性恶性肿瘤中速激肽和速激肽受体 mRNA 的表达，并与正常 B 细胞进行了比较。在人类 B 淋巴细胞中检测到 hHK-1 和 NK(1)受体的表达，但未检测到 SP 的表达，在慢性淋巴细胞白血病和非霍奇金淋巴瘤的 B 淋巴细胞中上调，而在急性淋巴细胞白血病中下调。此外，与 SP 相反，hHK-1 能够通过一种不依赖于 NK(1)受体的机制诱导人前 B 淋巴细胞的增殖。这些数据表明 hHK-1 在正常和病理性 B 淋巴细胞生成中具有作用，并为更好地理解导致淋巴细胞增殖性恶性肿瘤的生理病理机制开辟了道路。
