Jason L. Choy Laboratory of Single-Molecule Biophysics, University of California, Berkeley, 94720, USA.
Cell. 2011 Feb 18;144(4):480-97. doi: 10.1016/j.cell.2011.01.033.
The faithful relay and timely expression of genetic information depend on specialized molecular machines, many of which function as nucleic acid translocases. The emergence over the last decade of single-molecule fluorescence detection and manipulation techniques with nm and Å resolution and their application to the study of nucleic acid translocases are painting an increasingly sharp picture of the inner workings of these machines, the dynamics and coordination of their moving parts, their thermodynamic efficiency, and the nature of their transient intermediates. Here we present an overview of the main results arrived at by the application of single-molecule methods to the study of the main machines of the central dogma.
遗传信息的忠实传递和及时表达依赖于专门的分子机器，其中许多机器作为核酸转位酶发挥作用。过去十年中，单分子荧光检测和操纵技术的出现，分辨率达到纳米和埃，并将其应用于核酸转位酶的研究，正在为这些机器的内部工作机制、运动部件的动力学和协调性、热力学效率以及它们的瞬态中间体的性质描绘出一幅越来越清晰的画面。在这里，我们概述了将单分子方法应用于中心法则主要机器研究中所得到的主要结果。
