Zhou Ning, Hu Guangyuan, Mei Qi, Qiu Hong, Long Guoxian, Chen Chunli, Guoqing Hu
Cancer Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2011 Feb;31(1):62-66. doi: 10.1007/s11596-011-0151-7. Epub 2011 Feb 19.
The inhibitory effects of Endostar in combination with radiotherapy in BALB/c nude mice model of human CNE2 nasopharyngeal carcinoma and the mechanism were investigated. In nude mice model of CNE2 nasopharyngeal carcinoma, the inhibitory rate and the sensitizing enhancement ratio (E/O) were calculated according to the tumor volumes in different groups. The expression of microvascular density (MVD) in tumor tissues was examined by using immunohistochemistry staining. The transcription of VEGF gene was detected by using RT-PCR. The inhibitory rate in Endostar+ radiotherapy group was higher than in other groups. In Endostar+radiotherapy group, the tumor volume was significantly decreased and the E/O ratio was 2.335, suggesting that Endostar could be a radiosensitizer. The expression of MVD of tumor tissues in Endostar+radiotherapy group was reduced significantly. The expression of the MVD in treatment groups was significantly different from that in control group (P<0.05). Compared to other groups, VEGF mRNA expression in Endostar+radiotherapy group was decreased remarkably. Endostar in combination with radiotherapy significantly inhibited the growth of CNE2 tumor. The combination therapy decreased the expression of VEGF, and inhibited tumor angiogenesis and proliferation. When combined with radiotherapy, Endostar acted as a radiosensitizer.
研究了恩度联合放疗对人CNE2鼻咽癌BALB/c裸鼠模型的抑制作用及其机制。在CNE2鼻咽癌裸鼠模型中，根据不同组的肿瘤体积计算抑制率和增敏增强比（E/O）。采用免疫组化染色检测肿瘤组织中微血管密度（MVD）的表达。采用RT-PCR检测VEGF基因的转录。恩度+放疗组的抑制率高于其他组。恩度+放疗组肿瘤体积明显减小，E/O比值为2.335，提示恩度可作为放射增敏剂。恩度+放疗组肿瘤组织MVD表达明显降低。各治疗组MVD表达与对照组相比差异有统计学意义（P<0.05）。与其他组相比，恩度+放疗组VEGF mRNA表达明显降低。恩度联合放疗可显著抑制CNE2肿瘤生长。联合治疗降低了VEGF表达，抑制了肿瘤血管生成和增殖。恩度与放疗联合应用时可作为放射增敏剂。
