Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, 90610-000, Porto Alegre, RS, Brasil.
Mem Inst Oswaldo Cruz. 2011 Feb;106(1):51-5. doi: 10.1590/s0074-02762011000100008.
Biofilm production is an important mechanism that allows microbes to escape host defences and antimicrobial therapy. Vancomycin has been used largely for the treatment of methicillin-resistant staphylococcal infections. Here, we determined the minimal inhibitory concentration (MIC) and minimal biofilm eradication concentration (MBEC) for 82 Staphylococcus species isolated from central venous catheters (CVC). Our results showed that the 41 strong and moderate-biofilm-producing isolates presented a higher MBEC/MIC ratio for vancomycin than the 24 weak-biofilm-producing isolates, illustrating the importance of biofilm production ability and the difficulty in treating biofilm-related infections. The MBEC was significantly higher in moderate-biofilm-producing isolates than in weak-biofilm-producing isolates (p < 0.001) and in strong-biofilm-producing isolates than in weak-biofilm-producing isolates (p = 0.001). The correlation between the MIC and the MBEC was poor. Based on our results, we recommend that bacterial biofilms be suspected in all cases of CVC infection.
生物膜的产生是微生物逃避宿主防御和抗菌治疗的重要机制。万古霉素主要用于治疗耐甲氧西林葡萄球菌感染。在这里，我们确定了从中心静脉导管（CVC）中分离出的 82 种金黄色葡萄球菌的最小抑菌浓度（MIC）和最小生物膜清除浓度（MBEC）。我们的结果表明，41 株强和中度生物膜产生株的万古霉素 MBEC/MIC 比值高于 24 株弱生物膜产生株，这表明生物膜产生能力的重要性和治疗生物膜相关感染的难度。与弱生物膜产生株相比，中度生物膜产生株的 MBEC 明显更高（p < 0.001），与弱生物膜产生株相比，强生物膜产生株的 MBEC 也更高（p = 0.001）。MIC 和 MBEC 之间的相关性很差。根据我们的结果，我们建议在所有 CVC 感染病例中怀疑存在细菌生物膜。
