三聚体 gp120 表位模拟物与 T 辅助肽缀合以提高抗原性。
Synthesis of a trimeric gp120 epitope mimic conjugated to a T-helper peptide to improve antigenicity.
机构信息
Chemistry Department, Campus Mass Spectrometry Facility, University of California at Davis, 1 Shields Avenue, Davis, California 95616, United States.
出版信息
J Am Chem Soc. 2011 Mar 16;133(10):3230-3. doi: 10.1021/ja1083915. Epub 2011 Feb 22.
Abstract
A fully synthetic trivalent mimotope of gp120 conjugated to pan allelic HLA DR binding epitope was prepared using solid-phase peptide synthesis and optimized copper-catalyzed azide-alkyne cycloaddition. The methodology efficiently provides chemically uniform heteromultimeric peptide constructs with enhanced binding, avidity, and specificity toward an established HIV-neutralizing human antibody, MAb b12. The versatile synthetic strategy serves as a powerful platform for the development of synthetic peptides as potential HIV-1 vaccine candidates.
摘要
使用固相肽合成和优化的铜催化叠氮-炔环加成反应，制备了完全合成的 gp120 三聚体模拟物，该模拟物与所有等位基因 HLA-DR 结合表位缀合。该方法有效地提供了化学均匀的异多聚体肽构建体，与已建立的 HIV 中和人抗体 MAb b12 具有增强的结合、亲和力和特异性。这种多功能的合成策略是开发作为潜在 HIV-1 疫苗候选物的合成肽的强大平台。
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