Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Clin Infect Dis. 2011 Jan 1;52 Suppl 1:S102-8. doi: 10.1093/cid/ciq026.
We report shedding duration of 2009 pandemic influenza A (pH1N1) virus from a school-associated outbreak in Pennsylvania during May through June 2009. Outbreak-associated students or household contacts with influenza-like illness (ILI) onset within 7 days of interview were recruited. Nasopharyngeal specimens, collected every 48 hours until 2 consecutive nonpositive tests, underwent real-time reverse transcriptase polymerase chain reaction (rRT-PCR) and culture for pH1N1 virus. Culture-positive specimens underwent virus titrations. Twenty-six (median age, 8 years) rRT-PCR-positive persons, for pH1N1 virus, were included in analysis. Median shedding duration from fever onset by rRT-PCR was 6 days (range, 1-13) and 5 days (range, 1-7) by culture. Following fever resolution virus was isolated for a median of 2 days (range, 0-5). Highest and lowest virus titers detected, 2 and 5 days following fever onset, were 3.2 and 1.2 log(10) TCID(50)/mL respectively. Overall, shedding duration in children and adults were similar to seasonal influenza viruses.
我们报告了 2009 年宾夕法尼亚州一起学校相关暴发疫情中，2009 年甲型 H1N1 流感病毒的脱落持续时间。招募了在访谈后 7 天内出现流感样疾病（ILI）的暴发相关学生或家庭接触者。采集鼻咽标本，每 48 小时采集一次，直至连续两次检测均为阴性，然后进行实时逆转录聚合酶链反应（rRT-PCR）和 pH1N1 病毒培养。对培养阳性标本进行病毒滴定。共纳入 26 名（中位年龄 8 岁）rRT-PCR 检测 pH1N1 病毒阳性的人进行分析。rRT-PCR 检测从发热开始到病毒脱落的中位时间为 6 天（范围 1-13 天），通过培养为 5 天（范围 1-7 天）。发热消退后，病毒平均分离 2 天（范围 0-5 天）。发热后第 2 和第 5 天检测到的最高和最低病毒滴度分别为 3.2 和 1.2 log(10) TCID(50)/mL。总体而言，儿童和成人的脱落持续时间与季节性流感病毒相似。
