Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Japan.
J Radiat Res. 2011;52(2):185-92. doi: 10.1269/jrr.10116. Epub 2011 Feb 19.
The increasing use of mobile phones has aroused public concern regarding the potential health risks of radiofrequency (RF) fields. We investigated the effects of exposure to RF fields (2.45 GHz, continuous wave) at specific absorption rate (SAR) of 1, 5, and 10 W/kg for 1, 4, and 24 h on gene expression in a normal human glial cell line, SVGp12, using DNA microarray. Microarray analysis revealed 23 assigned gene spots and 5 non-assigned gene spots as prospective altered gene spots. Twenty-two genes out of the 23 assigned gene spots were further analyzed by reverse transcription-polymerase chain reaction to validate the results of microarray, and no significant alterations in gene expression were observed. Under the experimental conditions used in this study, we found no evidence that exposure to RF fields affected gene expression in SVGp12 cells.
随着手机使用量的增加，人们越来越关注射频（RF）场可能对健康造成的危害。我们使用 DNA 微阵列研究了在特定吸收率（SAR）为 1、5 和 10 W/kg 时，暴露于 RF 场（2.45 GHz，连续波）1、4 和 24 小时对正常人类神经胶质细胞系 SVGp12 中基因表达的影响。微阵列分析显示，23 个指定基因斑点和 5 个未指定基因斑点作为潜在改变的基因斑点。在微阵列中，进一步通过逆转录-聚合酶链反应分析了 23 个指定基因斑点中的 22 个基因，以验证微阵列的结果，未观察到基因表达的显著变化。在本研究中使用的实验条件下，我们没有发现暴露于 RF 场会影响 SVGp12 细胞中基因表达的证据。
